Wet Mount Evaluation of Sperm

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


The use of phase contrast and differential interference contrast (DIC) microscopy enhances the observation of structural detail within a cell. Wet mount preparation, as used in phase-contrast and DIC microscopy, causes less physical damage to spermatozoa. Phase-contrast and DIC techniques make use of phase and amplitude modulations or differential modulation of the light, respectively, to obtain contrast. Phase contrast produces a flatter image with darker cellular features ranging from black to white, whereas DIC produces an image with more uniform grays. This chapter illustrates a procedure for preparation of a wet mount, and outlines some necessary condition for evaluation of stallion spermatozoa.

Original languageEnglish
Title of host publicationEquine Reproductive Procedures
Number of pages3
ISBN (Electronic)9781118904398
ISBN (Print)9780470960394
StatePublished - Aug 25 2014


  • Differential interference contrast (DIC) microscopy
  • Phase contrast microscopy
  • Stallion spermatozoa
  • Wet mount evaluation


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