Validation of a field-ready handheld meter for plasma Beta-hydroxybutyrate analysis

Amie S Sommers, W Alice Boyle, Liam McGuire

Research output: Contribution to journalArticle

Abstract

Plasma metabolite analysis is frequently used to assess the energetic state and energy intake of birds. Plasma β‐hydroxybutyrate (BUTY) is a common metabolite used in these studies, and is correlated with fasting and mass loss. BUTY is typically quantified in laboratory assays that are costly, time‐consuming, and prone to human error. We tested the accuracy and precision of a field‐ready handheld BUTY meter. We compared BUTY concentration values obtained in the laboratory and with the handheld meter in plasma samples from 19 Grasshopper Sparrows (Ammodramus savannarum), and assessed precision with repeat analysis of a single sample. The handheld meter reported BUTY concentrations in < 2 min, was highly precise, and as accurate as the laboratory assay method—all ideal for field conditions. Collecting blood samples for laboratory analysis, particularly from remote field sites, involves a series of risks and challenges for permitting or the logistics of storage and shipping, as well as t
Original languageEnglish
Pages (from-to)399 - 404
JournalJournal of Field Ornithology
DOIs
StatePublished - Dec 15 2017

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