Utility of chromosomal position of heterochromatin as a biomarker of radiation-induced genetic damage: A study of Chornobyl voles (Microtus sp.)

Lara E. Wiggins; Ronald A. Van Den Bussche; Meredith J. Hamilton; Ronald K. Chesser; Robert J. Baker

doi:10.1023/A:1015466530422

Utility of chromosomal position of heterochromatin as a biomarker of radiation-induced genetic damage: A study of Chornobyl voles (Microtus sp.)

Lara E. Wiggins, Ronald A. Van Den Bussche, Meredith J. Hamilton, Ronald K. Chesser, Robert J. Baker

Biological Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Biomarkers that effectively document effects of chronic multi-generational exposure to contaminated environments on chromosomes would be valuable in risk assessment, remediation, and environmental decisions. Native, free-ranging populations of voles inhabiting the highly radioactive regions surrounding Reactor 4 of the Chornobyl Nuclear Power Station provide a model system to evaluate biological and chromosomal effects of chronic multi-generational exposure to radioactivity and other reactor meltdown-related pollutants. Here, we explore the utility of heterochromatic elements as potentially informative biomarkers for genetic damage in voles from the radioactive environments surrounding Chornobyl. We analyzed chromosomal positions of heterochromatin from Microtus arvalis and M. rossiaemeridionalis using fluorescent in situ hybridization. Although intrapopulational variation existed in chromosomal position and abundance of heterochromatin, none of that variation could be assigned to environmental exposure.

Original language	English
Pages (from-to)	147-154
Number of pages	8
Journal	Ecotoxicology
Volume	11
Issue number	3
DOIs	https://doi.org/10.1023/A:1015466530422
State	Published - 2002

Keywords

Biomarkers
Chernobyl
Chornobyl
Heterochromatin
Microtus

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10.1023/A:1015466530422

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title = "Utility of chromosomal position of heterochromatin as a biomarker of radiation-induced genetic damage: A study of Chornobyl voles (Microtus sp.)",

abstract = "Biomarkers that effectively document effects of chronic multi-generational exposure to contaminated environments on chromosomes would be valuable in risk assessment, remediation, and environmental decisions. Native, free-ranging populations of voles inhabiting the highly radioactive regions surrounding Reactor 4 of the Chornobyl Nuclear Power Station provide a model system to evaluate biological and chromosomal effects of chronic multi-generational exposure to radioactivity and other reactor meltdown-related pollutants. Here, we explore the utility of heterochromatic elements as potentially informative biomarkers for genetic damage in voles from the radioactive environments surrounding Chornobyl. We analyzed chromosomal positions of heterochromatin from Microtus arvalis and M. rossiaemeridionalis using fluorescent in situ hybridization. Although intrapopulational variation existed in chromosomal position and abundance of heterochromatin, none of that variation could be assigned to environmental exposure.",

keywords = "Biomarkers, Chernobyl, Chornobyl, Heterochromatin, Microtus",

author = "Wiggins, {Lara E.} and {Van Den Bussche}, {Ronald A.} and Hamilton, {Meredith J.} and Chesser, {Ronald K.} and Baker, {Robert J.}",

note = "Funding Information: Elena Bundova, Andrew DeWoody, Sergei Gaschak, Galena Rudenskaya, and Derrick Sugg provided assistance in the collection and preparation of the specimens utilized in this study. Thanks to Brenda Rodgers and Deidre Parish for their assistance with figure preparation and for critically reviewing this manuscript. Support for this research was provided (in part) by a grant from the Howard Hughes Medical Institute through the Biological Sciences Undergraduate Education Program at Texas Tech University, the Clark Scholars Program, and by contract DE-FC09-96SR18546 between the United States Department of Energy and the University of Georgia Savannah River Ecology Laboratory.",

year = "2002",

doi = "10.1023/A:1015466530422",

language = "English",

volume = "11",

pages = "147--154",

journal = "Ecotoxicology",

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AU - Van Den Bussche, Ronald A.

AU - Hamilton, Meredith J.

AU - Chesser, Ronald K.

AU - Baker, Robert J.

N1 - Funding Information: Elena Bundova, Andrew DeWoody, Sergei Gaschak, Galena Rudenskaya, and Derrick Sugg provided assistance in the collection and preparation of the specimens utilized in this study. Thanks to Brenda Rodgers and Deidre Parish for their assistance with figure preparation and for critically reviewing this manuscript. Support for this research was provided (in part) by a grant from the Howard Hughes Medical Institute through the Biological Sciences Undergraduate Education Program at Texas Tech University, the Clark Scholars Program, and by contract DE-FC09-96SR18546 between the United States Department of Energy and the University of Georgia Savannah River Ecology Laboratory.

PY - 2002

Y1 - 2002

N2 - Biomarkers that effectively document effects of chronic multi-generational exposure to contaminated environments on chromosomes would be valuable in risk assessment, remediation, and environmental decisions. Native, free-ranging populations of voles inhabiting the highly radioactive regions surrounding Reactor 4 of the Chornobyl Nuclear Power Station provide a model system to evaluate biological and chromosomal effects of chronic multi-generational exposure to radioactivity and other reactor meltdown-related pollutants. Here, we explore the utility of heterochromatic elements as potentially informative biomarkers for genetic damage in voles from the radioactive environments surrounding Chornobyl. We analyzed chromosomal positions of heterochromatin from Microtus arvalis and M. rossiaemeridionalis using fluorescent in situ hybridization. Although intrapopulational variation existed in chromosomal position and abundance of heterochromatin, none of that variation could be assigned to environmental exposure.

AB - Biomarkers that effectively document effects of chronic multi-generational exposure to contaminated environments on chromosomes would be valuable in risk assessment, remediation, and environmental decisions. Native, free-ranging populations of voles inhabiting the highly radioactive regions surrounding Reactor 4 of the Chornobyl Nuclear Power Station provide a model system to evaluate biological and chromosomal effects of chronic multi-generational exposure to radioactivity and other reactor meltdown-related pollutants. Here, we explore the utility of heterochromatic elements as potentially informative biomarkers for genetic damage in voles from the radioactive environments surrounding Chornobyl. We analyzed chromosomal positions of heterochromatin from Microtus arvalis and M. rossiaemeridionalis using fluorescent in situ hybridization. Although intrapopulational variation existed in chromosomal position and abundance of heterochromatin, none of that variation could be assigned to environmental exposure.

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Utility of chromosomal position of heterochromatin as a biomarker of radiation-induced genetic damage: A study of Chornobyl voles (Microtus sp.)

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