Using image-based flow cytometry to monitor satellite cells proliferation and differentiation in vitro

Hui Ying Luk, Brian K. McFarlin, Jakob L. Vingren

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Muscle satellite cells can proliferate and differentiate into myocytes; this biological function has important implications for muscle development, aging, repair, and wasting diseases. Established analytical methods, including western blotting, PCR, and immunohistochemistry, has been used to characterize the stages of satellite cells growth and development but there is currently a lack of methods to simultaneously monitor cell proliferation and identify changes in cell properties (i.e., expression of myogenic regulatory factors) for each individual cell during the progression to become myocytes. Image-based flow cytometry has the capability to simultaneously obtain morphometric data, monitor cell division, and detect expression of multiple proteins of interest. In this article we demonstrate the use of image-based flow cytometry and cell-trace dye to monitor satellite cells proliferation and to identify different stages of satellite cell differentiation.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalMethods
Volume112
DOIs
StatePublished - Jan 1 2017

Keywords

  • Differentiation
  • MyoD
  • Myoblasts
  • Myocytes
  • Pax7
  • Proliferation

Fingerprint Dive into the research topics of 'Using image-based flow cytometry to monitor satellite cells proliferation and differentiation in vitro'. Together they form a unique fingerprint.

  • Cite this