Using hydrothermal method to prepare reduced graphene-hemin electrochemical biosensor for tyrosine detection

Junhua Wei, Jenny Qiu

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Hemin immobilized reduced graphene(HGN) has been investigated to be an outstanding enzymatic catalysis in detection important molecular recently. In this work, two "clean" methods to prepare HGN through π- π stack were charactered by UV-vis spectra, TEM images, and 5- potential. The enzymatic catalysis of both materials was compared by catalytic hydrogen peroxide to oxidize pyrogallol. The colorimetric result shows HGN attached before reduction has stronger catalytic ability than the one after reduction. The optimized HGN was then used as an electrochemical biosensor to determine L-tyrosine levels. The cyclic voltammetry (CV) tests were carried out for the bare glass carbon electrode (GCE), and the optimized hemin-reduced graphene electrode (HGN1/GCE). The HGN1/GCE based biosensor exhibits a Tyrosine detection linear range from 5× 10-7 M to 4×10-5 M with a detection limitation of 7.5× 10-8 M at signal noise ratio (S/N) of 3. In comparison with other biosensor, electrochemical biosensors are easy-fabricated, easy-controlled, and cost-effective. Compared with other materials, the hemin- reduced graphene based biosensors demonstrate higher stability, a broader detection linear range, and better detection sensitivity. The study of oxidation scheme reveals that reduced graphene enhanced the electron transfer between electrode and hemin. Meanwhile, the hemin groups effectively electrocatalyzed the oxidation of tyrosine. This study contributes to a widespread clinical application of nanomaterial based biosensor devices with a broader detection linear range, improved stability, enhanced sensitivity, and reduced costs.

Original languageEnglish
Title of host publicationCarbon Nanomaterials
PublisherMaterials Research Society
Number of pages7
ISBN (Print)9781632660985
StatePublished - 2013
Event2012 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 25 2012Nov 30 2012

Publication series

NameMaterials Research Society Symposium Proceedings
ISSN (Print)0272-9172


Conference2012 MRS Fall Meeting
Country/TerritoryUnited States
CityBoston, MA


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