Using hydrothermal method to prepare reduced graphene-hemin electrochemical biosensor for tyrosine detection

Junhua Wei, Jenny Qiu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Hemin immobilized reduced graphene(HGN) has been investigated to be an outstanding enzymatic catalysis in detection important molecular recently. In this work, two "clean" methods to prepare HGN through π- π stack were charactered by UV-vis spectra, TEM images, and 5- potential. The enzymatic catalysis of both materials was compared by catalytic hydrogen peroxide to oxidize pyrogallol. The colorimetric result shows HGN attached before reduction has stronger catalytic ability than the one after reduction. The optimized HGN was then used as an electrochemical biosensor to determine L-tyrosine levels. The cyclic voltammetry (CV) tests were carried out for the bare glass carbon electrode (GCE), and the optimized hemin-reduced graphene electrode (HGN1/GCE). The HGN1/GCE based biosensor exhibits a Tyrosine detection linear range from 5× 10-7 M to 4×10-5 M with a detection limitation of 7.5× 10-8 M at signal noise ratio (S/N) of 3. In comparison with other biosensor, electrochemical biosensors are easy-fabricated, easy-controlled, and cost-effective. Compared with other materials, the hemin- reduced graphene based biosensors demonstrate higher stability, a broader detection linear range, and better detection sensitivity. The study of oxidation scheme reveals that reduced graphene enhanced the electron transfer between electrode and hemin. Meanwhile, the hemin groups effectively electrocatalyzed the oxidation of tyrosine. This study contributes to a widespread clinical application of nanomaterial based biosensor devices with a broader detection linear range, improved stability, enhanced sensitivity, and reduced costs.

Original languageEnglish
Title of host publicationCarbon Nanomaterials
PublisherMaterials Research Society
Pages180-186
Number of pages7
ISBN (Print)9781632660985
DOIs
StatePublished - 2013
Event2012 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 25 2012Nov 30 2012

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1505
ISSN (Print)0272-9172

Conference

Conference2012 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period11/25/1211/30/12

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    Wei, J., & Qiu, J. (2013). Using hydrothermal method to prepare reduced graphene-hemin electrochemical biosensor for tyrosine detection. In Carbon Nanomaterials (pp. 180-186). (Materials Research Society Symposium Proceedings; Vol. 1505). Materials Research Society. https://doi.org/10.1557/opl.2013.524