Uptake of 17Β-trenbolone and subsequent metabolite trendione by the pinto bean plant (Phaseolus vulgaris)

B. R. Blackwell, A. Karnjanapiboonwong, T. A. Anderson, P. N. Smith

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Manure from livestock feeding operations is commonly applied to agricultural fields as an alternative to commercial fertilizers. Trenbolone acetate (TbA) is a synthetic growth promoter frequently utilized in beef cattle feeding operations. Metabolites of TbA can be present in manure and subsequently applied to fields. Fate ofTbA metabolites 17. Β-trenbolone (17. ΒTb), 17. α-trenbolone (17. αTb), and trendione (TbO) have been assessed in manure and soils, but plant uptake in agricultural fields is not fully understood. The objective of this study was to investigate potential plant uptake and biotransformation of 17. ΒTb using the pinto bean plant (Phaseolus vulgaris). Vegetated (n=20) and control sands (n=16) were amended with 17. ΒTb at a level of 1. μg/g once per week for a total of four weeks. Sand, above-ground plant portion and below-ground plant portion were collected each week and then analyzed for 17. ΒTb, 17. αTb, and TbO. By week four, low concentrations of 17. ΒTb (10±4.9. μg/g fresh weight) were taken up into the roots of plants and, to a much lesser extent, translocated throughout the plant (0.04±0.02. μg/g fresh weight). Extensive transformation of 17. ΒTb to the metabolite trendione (TbO) occurred in vegetated sand, while minimal TbO was detected in control sand. These results suggest the biotransformation of 17. ΒTb to TbO is predominantly through microbial degradation. Trenbolone (Tb) metabolites can then be taken up into plants but remain concentrated in the roots with only slight translocation to above ground portions of the plant. After four weeks, maximum observed concentrations of total Tb (parent+metabolites) in fresh plant tissues were 33.0. μg/g in roots and 0.25. μg/g in leaves. No phytotoxicity was observed to pinto bean plants throughout the four week study.

Original languageEnglish
Pages (from-to)110-114
Number of pages5
JournalEcotoxicology and Environmental Safety
Volume85
DOIs
StatePublished - Nov 1 2012

Keywords

  • Bioconcentration factor (BCF)
  • Plant uptake
  • Steroid
  • Trenbolone

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