Uniform yeast cell assembly via microfluidics

Ya Wen Chang, Peng He, Samantha M. Marquez, Zhengdong Cheng

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

This paper reports the use of microfluidic approaches for the fabrication of yeastosomes (yeast-celloidosomes) based on self-assembly of yeast cells onto liquid-solid or liquid-gas interfaces. Precise control over fluidic flows in droplet- and bubble-forming microfluidic devices allows production of monodispersed, size-selected templates. The general strategy to organize and assemble living cells is to tune electrostatic attractions between the template (gel or gas core) and the cells via surface charging. Layer-by-Layer (LbL) polyelectrolyte deposition was employed to invert or enhance charges of solid surfaces. We demonstrated the ability to produce high-quality, monolayer-shelled yeastosome structures under proper conditions when sufficient electrostatic driving forces are present. The combination of microfluidic fabrication with cell self-assembly enables a versatile platform for designing synthetic hierarchy bio-structures.

Original languageEnglish
Article number024118
JournalBiomicrofluidics
Volume6
Issue number2
DOIs
StatePublished - Apr 26 2012

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