TY - JOUR
T1 - Two PGPR strains from the rhizosphere of Haloxylon ammodendron promoted growth and enhanced drought tolerance of ryegrass
AU - He, Aolei
AU - Niu, Shuqi
AU - Yang, Di
AU - Ren, Wei
AU - Zhao, Lingyu
AU - Sun, Yunya
AU - Meng, Laisheng
AU - Zhao, Qi
AU - Paré, Paul W.
AU - Zhang, Jinlin
N1 - Funding Information:
This work was financially supported by the National Key Research and Development Program of China (grant No. 2019YFC0507703 ), the National Natural Science Foundation of China (grant No. 31222053 and 32071875 ) and the Science and Technology Support Program of Gansu Province, China (grant No. 1604NKCA077 ).
Publisher Copyright:
© 2021 Elsevier Masson SAS
PY - 2021/4
Y1 - 2021/4
N2 - Haloxylon ammodendron, a typical xerophyte, tolerates various abiotic stresses and is widely distributed in desert areas. Two PGPR strains, Bacillus sp. WM13-24 and Pseudomonas sp. M30-35, were previously isolated from the rhizosphere of H. ammodendron in Tengger Desert, Gansu province, northwest China. The aim of this study was to investigate the role of M30-35 and WM13-24 in drought stress alleviation of ryegrass (Lolium perenne L.). Under normal condition, both M30-35 and WM13-24 increased shoot fresh and dry weight, chlorophyll content, total nitrogen and phosphorus contents and altered phytohormone distribution compared to control. Moreover, after 7 days of drought stress, WM13–24 and M30-35 enhanced photosynthetic capacity, relative water content, the activities of catalase (CAT) and peroxidase (POD) and proline content, resulted in decreased malondialdehyde (MDA) content, relative membrane permeability (RMP) and H2O2 accumulation; interestingly, the two strains decreased ABA content in leaves. This study demonstrated that the two PGPR strains promoted ryegrass growth and root development via regulating plant hormone distribution and enhanced drought tolerance of ryegrass through improving the activities of antioxidant enzymes, regulating ABA signaling and maintaining plant growth. Our results indicated that PGPR strains from rhizosphere of the desert plant species could be considered as promising bioinoculants for grass plants.
AB - Haloxylon ammodendron, a typical xerophyte, tolerates various abiotic stresses and is widely distributed in desert areas. Two PGPR strains, Bacillus sp. WM13-24 and Pseudomonas sp. M30-35, were previously isolated from the rhizosphere of H. ammodendron in Tengger Desert, Gansu province, northwest China. The aim of this study was to investigate the role of M30-35 and WM13-24 in drought stress alleviation of ryegrass (Lolium perenne L.). Under normal condition, both M30-35 and WM13-24 increased shoot fresh and dry weight, chlorophyll content, total nitrogen and phosphorus contents and altered phytohormone distribution compared to control. Moreover, after 7 days of drought stress, WM13–24 and M30-35 enhanced photosynthetic capacity, relative water content, the activities of catalase (CAT) and peroxidase (POD) and proline content, resulted in decreased malondialdehyde (MDA) content, relative membrane permeability (RMP) and H2O2 accumulation; interestingly, the two strains decreased ABA content in leaves. This study demonstrated that the two PGPR strains promoted ryegrass growth and root development via regulating plant hormone distribution and enhanced drought tolerance of ryegrass through improving the activities of antioxidant enzymes, regulating ABA signaling and maintaining plant growth. Our results indicated that PGPR strains from rhizosphere of the desert plant species could be considered as promising bioinoculants for grass plants.
KW - Drought tolerance
KW - Growth promotion
KW - Haloxylon ammodendron
KW - PGPR
KW - Rhizosphere
KW - Ryegrass
UR - http://www.scopus.com/inward/record.url?scp=85100637007&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2021.02.003
DO - 10.1016/j.plaphy.2021.02.003
M3 - Article
C2 - 33578287
AN - SCOPUS:85100637007
SN - 0981-9428
VL - 161
SP - 74
EP - 85
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
ER -