Transgenic maize plants of tropical and subtropical genotypes obtained from calluses containing organogenic and embryogenic-like structures derived from shoot tips

A. O'Connor-Sánchez, J. L. Cabrera-Ponce, M. Valdez-Melara, P. Téllez-Rodríguez, J. L. Pons-Hernández, L. Herrera-Estrella

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

A highly efficient system for the production of transgenic maize plants starting from tropical and subtropical genotypes was developed. The method is based on particle bombardment of organogenic calli derived from shoot tips. Six tropical maize genotypes were successfully transformed and regenerated using this protocol. Genetic transformation was confirmed by Southern blot analysis of T0 plants and segregation analysis of the resistance marker in the T1 progeny. Plant transfer into the greenhouse was 100% successful, and no problems of fertility were observed with the transgenic plants produced with this transformation protocol.

Original languageEnglish
Pages (from-to)302-312
Number of pages11
JournalPlant Cell Reports
Volume21
Issue number4
DOIs
StatePublished - 2002

Keywords

  • Cereal transformation
  • Organogenesis
  • Plant regeneration
  • Shoot meristem
  • Transgenic maize

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