@article{54959682d78349c587a4f83798817f9f,
title = "Transgenic central american, west african and asian elite rice varieties resulting from particle bombardment of foreign dna into mature seed-derived expiants utilizing three different bombardment devices",
abstract = "We developed a novel system based on particle bombardment for the transfer of foreign genes into eight important rice varieties grown in Central America, West Africa and Asia. Novel features of this improved protocol, which we believe will replace alternative systems currently utilizing immature embryos, embryog{\'e}nie cell lines or suspension cultures include: (a) bombardment of target tissue 5d after seed germination; (b) no requirement to select for embryog{\'e}nie callus prior to bombardment; (c) high transformation frequencies (up to 14%); (d) ability to recover transgenic material at similar frequencies irrespective of bombardment devices used. Southern blot analysis and enzymatic assays in primary transformants and progeny confirmed the stable integration and expression of introduced transgenes in all engineered cultivars.",
keywords = "Oryza saliva, Particle bombardment, Transformation, Transgenic rice",
author = "M. Valdez and Cabrera-Ponce, {J. L.} and D. Sudhakar and L. Herrera-Estrella and P. Christou",
note = "Funding Information: We thank Silvia Solis, Reinhardt Acuna and Marta Martinez for technical assistance, Antonio Cisneros for the photographic work, Guadalupe Sanchez and Julie Smith for maintaining plants in the glasshouse. Marta Valdez is grateful to UNU}Biolac and to UNESCO for visiting scientist fellowships at CINVESTAV and the JIC. We also thank our colleagues at the JIC for useful discussions and support. This work was supported in part by a grant from the Rockefeller Foundation to Luis Herrera-Estrella, and by the University of Costa Rica (Project NBO 111-92-570). D. Sudhakar and P. Christou thank the Rockefeller Foundation for financial support. The JIC is supported by a grant in aid from the BBSRC, UK.",
year = "1998",
doi = "10.1006/anbo.1998.0753",
language = "English",
volume = "82",
pages = "795--801",
journal = "Annals of Botany",
issn = "0305-7364",
number = "6",
}