Transformation of four pathogenic Phytophthora spp by microprojectile bombardment on intact mycelia

Ana M. Bailey, Gilda L. Mena, Luis Herrera-Estrella

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the β-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric β-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.

Original languageEnglish
Pages (from-to)42-46
Number of pages5
JournalCurrent Genetics
Volume23
Issue number1
DOIs
StatePublished - Jan 1993

Keywords

  • Biolistic
  • Phytophthora
  • Plant promoter
  • Transformation
  • β-glucuronidase

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