Transformation of four pathogenic Phytophthora spp by microprojectile bombardment on intact mycelia

Ana M. Bailey, Gilda L. Mena, Luis Herrera-Estrella

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the β-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric β-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.

Original languageEnglish
Pages (from-to)42-46
Number of pages5
JournalCurrent Genetics
Volume23
Issue number1
DOIs
StatePublished - Jan 1993

Keywords

  • Biolistic
  • Phytophthora
  • Plant promoter
  • Transformation
  • β-glucuronidase

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