Transformation of four pathogenic Phytophthora spp by microprojectile bombardment on intact mycelia

Ana M. Bailey; Gilda L. Mena; Luis Herrera-Estrella

doi:10.1007/BF00336748

Transformation of four pathogenic Phytophthora spp by microprojectile bombardment on intact mycelia

Ana M. Bailey, Gilda L. Mena, Luis Herrera-Estrella

Plant & Soil Science

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the β-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric β-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.

Original language	English
Pages (from-to)	42-46
Number of pages	5
Journal	Current Genetics
Volume	23
Issue number	1
DOIs	https://doi.org/10.1007/BF00336748
State	Published - Jan 1993

Keywords

Biolistic
Phytophthora
Plant promoter
Transformation
β-glucuronidase

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abstract = "Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the β-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric β-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.",

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AU - Mena, Gilda L.

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N2 - Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the β-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric β-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.

AB - Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the β-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric β-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.

KW - Biolistic

KW - Phytophthora

KW - Plant promoter

KW - Transformation

KW - β-glucuronidase

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JF - Current Genetics

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ER -

Transformation of four pathogenic Phytophthora spp by microprojectile bombardment on intact mycelia

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Keywords

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