Transfer of the maize transposable element MU1 into Arabidopsis thaliana

Hong Zhang; Chris R. Somerville

doi:10.1016/0168-9452(87)90086-0

Transfer of the maize transposable element MU1 into Arabidopsis thaliana

Hong Zhang, Chris R. Somerville

Biological Sciences

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The maize transposable element Mu1 was transferred to Arabidopsis thaliana by Ti plasmid-mediated transformation and a fertile line containing Mu1 was regenerated. Southern analysis of transformed tissue indicated that the Mu1 DNA remained entirely within a segment of T-DNA during three sexual generations. The results of a search for spontaneous mutations in a large number of Mu1-containing seedlings suggests that the presence of Mu1 did not cause a major increase in the spontaneous mutation frequency.

Original language	English
Pages (from-to)	165-173
Number of pages	9
Journal	Plant Science
Volume	48
Issue number	3
DOIs	https://doi.org/10.1016/0168-9452(87)90086-0
State	Published - 1987

Keywords

Arabidopsis thaliana
Robertson's Mutator
Ti plasmid
plant regeneration
transformation
transposable element

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10.1016/0168-9452(87)90086-0

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title = "Transfer of the maize transposable element MU1 into Arabidopsis thaliana",

abstract = "The maize transposable element Mu1 was transferred to Arabidopsis thaliana by Ti plasmid-mediated transformation and a fertile line containing Mu1 was regenerated. Southern analysis of transformed tissue indicated that the Mu1 DNA remained entirely within a segment of T-DNA during three sexual generations. The results of a search for spontaneous mutations in a large number of Mu1-containing seedlings suggests that the presence of Mu1 did not cause a major increase in the spontaneous mutation frequency.",

keywords = "Arabidopsis thaliana, Robertson's Mutator, Ti plasmid, plant regeneration, transformation, transposable element",

author = "Hong Zhang and Somerville, {Chris R.}",

note = "Funding Information: We thank R. Horsch for advice concerning the transformation of Arabidopsis and J. Schell, M. Freeling, W. Taylor, J. Bennetzen and S. Rogers for the generous gifts of plasmids. This work was supported in part by grants from the National Science Foundation (No. PCM8351595) and the U.S. Department of Energy (AC02-76ER01338).",

year = "1987",

doi = "10.1016/0168-9452(87)90086-0",

language = "English",

volume = "48",

pages = "165--173",

journal = "Plant Science",

issn = "0168-9452",

publisher = "Elsevier Publishing",

number = "3",

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T1 - Transfer of the maize transposable element MU1 into Arabidopsis thaliana

AU - Zhang, Hong

AU - Somerville, Chris R.

N1 - Funding Information: We thank R. Horsch for advice concerning the transformation of Arabidopsis and J. Schell, M. Freeling, W. Taylor, J. Bennetzen and S. Rogers for the generous gifts of plasmids. This work was supported in part by grants from the National Science Foundation (No. PCM8351595) and the U.S. Department of Energy (AC02-76ER01338).

PY - 1987

Y1 - 1987

N2 - The maize transposable element Mu1 was transferred to Arabidopsis thaliana by Ti plasmid-mediated transformation and a fertile line containing Mu1 was regenerated. Southern analysis of transformed tissue indicated that the Mu1 DNA remained entirely within a segment of T-DNA during three sexual generations. The results of a search for spontaneous mutations in a large number of Mu1-containing seedlings suggests that the presence of Mu1 did not cause a major increase in the spontaneous mutation frequency.

AB - The maize transposable element Mu1 was transferred to Arabidopsis thaliana by Ti plasmid-mediated transformation and a fertile line containing Mu1 was regenerated. Southern analysis of transformed tissue indicated that the Mu1 DNA remained entirely within a segment of T-DNA during three sexual generations. The results of a search for spontaneous mutations in a large number of Mu1-containing seedlings suggests that the presence of Mu1 did not cause a major increase in the spontaneous mutation frequency.

KW - Arabidopsis thaliana

KW - Robertson's Mutator

KW - Ti plasmid

KW - plant regeneration

KW - transformation

KW - transposable element

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U2 - 10.1016/0168-9452(87)90086-0

DO - 10.1016/0168-9452(87)90086-0

M3 - Article

AN - SCOPUS:38149144601

VL - 48

SP - 165

EP - 173

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 3

ER -

Transfer of the maize transposable element MU1 into Arabidopsis thaliana

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Keywords

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