Transfer of the maize transposable element MU1 into Arabidopsis thaliana

Hong Zhang; Chris R. Somerville

doi:10.1016/0168-9452(87)90086-0

Transfer of the maize transposable element MU1 into Arabidopsis thaliana

Hong Zhang, Chris R. Somerville

Biological Sciences

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The maize transposable element Mu1 was transferred to Arabidopsis thaliana by Ti plasmid-mediated transformation and a fertile line containing Mu1 was regenerated. Southern analysis of transformed tissue indicated that the Mu1 DNA remained entirely within a segment of T-DNA during three sexual generations. The results of a search for spontaneous mutations in a large number of Mu1-containing seedlings suggests that the presence of Mu1 did not cause a major increase in the spontaneous mutation frequency.

Original language	English
Pages (from-to)	165-173
Number of pages	9
Journal	Plant Science
Volume	48
Issue number	3
DOIs	https://doi.org/10.1016/0168-9452(87)90086-0
State	Published - 1987

Keywords

Arabidopsis thaliana
Robertson's Mutator
Ti plasmid
plant regeneration
transformation
transposable element

Access to Document

10.1016/0168-9452(87)90086-0

Fingerprint Dive into the research topics of 'Transfer of the maize transposable element MU1 into Arabidopsis thaliana'. Together they form a unique fingerprint.

Cite this

@article{06c4f46ba158419ab9a52fa235e76f3a,

title = "Transfer of the maize transposable element MU1 into Arabidopsis thaliana",

abstract = "The maize transposable element Mu1 was transferred to Arabidopsis thaliana by Ti plasmid-mediated transformation and a fertile line containing Mu1 was regenerated. Southern analysis of transformed tissue indicated that the Mu1 DNA remained entirely within a segment of T-DNA during three sexual generations. The results of a search for spontaneous mutations in a large number of Mu1-containing seedlings suggests that the presence of Mu1 did not cause a major increase in the spontaneous mutation frequency.",

keywords = "Arabidopsis thaliana, Robertson's Mutator, Ti plasmid, plant regeneration, transformation, transposable element",

author = "Hong Zhang and Somerville, {Chris R.}",

year = "1987",

doi = "10.1016/0168-9452(87)90086-0",

language = "English",

volume = "48",

pages = "165--173",

journal = "Plant Science",

issn = "0168-9452",

number = "3",

}

TY - JOUR

T1 - Transfer of the maize transposable element MU1 into Arabidopsis thaliana

AU - Zhang, Hong

AU - Somerville, Chris R.

PY - 1987

Y1 - 1987

N2 - The maize transposable element Mu1 was transferred to Arabidopsis thaliana by Ti plasmid-mediated transformation and a fertile line containing Mu1 was regenerated. Southern analysis of transformed tissue indicated that the Mu1 DNA remained entirely within a segment of T-DNA during three sexual generations. The results of a search for spontaneous mutations in a large number of Mu1-containing seedlings suggests that the presence of Mu1 did not cause a major increase in the spontaneous mutation frequency.

AB - The maize transposable element Mu1 was transferred to Arabidopsis thaliana by Ti plasmid-mediated transformation and a fertile line containing Mu1 was regenerated. Southern analysis of transformed tissue indicated that the Mu1 DNA remained entirely within a segment of T-DNA during three sexual generations. The results of a search for spontaneous mutations in a large number of Mu1-containing seedlings suggests that the presence of Mu1 did not cause a major increase in the spontaneous mutation frequency.

KW - Arabidopsis thaliana

KW - Robertson's Mutator

KW - Ti plasmid

KW - plant regeneration

KW - transformation

KW - transposable element

UR - http://www.scopus.com/inward/record.url?scp=38149144601&partnerID=8YFLogxK

U2 - 10.1016/0168-9452(87)90086-0

DO - 10.1016/0168-9452(87)90086-0

M3 - Article

AN - SCOPUS:38149144601

VL - 48

SP - 165

EP - 173

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 3

ER -