Transcriptome profiling characterizes phosphate deficiency effects on carbohydrate metabolism in rice leaves

Myoung Ryoul Park, So Hyeon Baek, Benildo G. De los Reyes, Song Joong Yun, Karl H. Hasenstein

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Phosphorus (P) is a structural component of nucleic acids and phospholipids and plays important roles in plant growth and development. P accumulation was significantly reduced (about 35%) in rice leaves from plants grown under low (32μM) P compared to 320μMP grown plants. Genome response to low P was examined using the rice 60K oligonucleotide DNA microarrays. At the threshold significance of |log 2| fold>2.0, 21,033 genes (about 33.7% of all genes on the microarray) were affected by P deficiency. Among all genes on the microarray, 4271 genes were sorted into 51 metabolic pathways. Low P affected 1494 (35.0%) genes and the largest category of genes was related to sucrose degradation to ethanol and lactate pathway. To survey the role of P in rice, 25 pathways were selected based on number of affected genes. Among these pathways, cytosolic glycolysis contained the least number of upregulated but most down-regulated genes. Low P decreased glucose, pyruvate and chlorophyll, and genes related to carbon metabolism and chlorophyllide a biosynthesis. However, sucrose and starch levels increased. These results indicate that P nutrition affects diverse metabolic pathways mostly related to glucose, pyruvate, sucrose, starch, and chlorophyll a.

Original languageEnglish
Pages (from-to)193-205
Number of pages13
JournalJournal of Plant Physiology
Volume169
Issue number2
DOIs
StatePublished - Jan 15 2012

Keywords

  • Carbohydrate
  • Metabolic pathway
  • Microarray
  • Phosphate
  • Rice

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