TY - JOUR
T1 - Transcriptome profiling characterizes phosphate deficiency effects on carbohydrate metabolism in rice leaves
AU - Park, Myoung Ryoul
AU - Baek, So Hyeon
AU - De los Reyes, Benildo G.
AU - Yun, Song Joong
AU - Hasenstein, Karl H.
PY - 2012/1/15
Y1 - 2012/1/15
N2 - Phosphorus (P) is a structural component of nucleic acids and phospholipids and plays important roles in plant growth and development. P accumulation was significantly reduced (about 35%) in rice leaves from plants grown under low (32μM) P compared to 320μMP grown plants. Genome response to low P was examined using the rice 60K oligonucleotide DNA microarrays. At the threshold significance of |log 2| fold>2.0, 21,033 genes (about 33.7% of all genes on the microarray) were affected by P deficiency. Among all genes on the microarray, 4271 genes were sorted into 51 metabolic pathways. Low P affected 1494 (35.0%) genes and the largest category of genes was related to sucrose degradation to ethanol and lactate pathway. To survey the role of P in rice, 25 pathways were selected based on number of affected genes. Among these pathways, cytosolic glycolysis contained the least number of upregulated but most down-regulated genes. Low P decreased glucose, pyruvate and chlorophyll, and genes related to carbon metabolism and chlorophyllide a biosynthesis. However, sucrose and starch levels increased. These results indicate that P nutrition affects diverse metabolic pathways mostly related to glucose, pyruvate, sucrose, starch, and chlorophyll a.
AB - Phosphorus (P) is a structural component of nucleic acids and phospholipids and plays important roles in plant growth and development. P accumulation was significantly reduced (about 35%) in rice leaves from plants grown under low (32μM) P compared to 320μMP grown plants. Genome response to low P was examined using the rice 60K oligonucleotide DNA microarrays. At the threshold significance of |log 2| fold>2.0, 21,033 genes (about 33.7% of all genes on the microarray) were affected by P deficiency. Among all genes on the microarray, 4271 genes were sorted into 51 metabolic pathways. Low P affected 1494 (35.0%) genes and the largest category of genes was related to sucrose degradation to ethanol and lactate pathway. To survey the role of P in rice, 25 pathways were selected based on number of affected genes. Among these pathways, cytosolic glycolysis contained the least number of upregulated but most down-regulated genes. Low P decreased glucose, pyruvate and chlorophyll, and genes related to carbon metabolism and chlorophyllide a biosynthesis. However, sucrose and starch levels increased. These results indicate that P nutrition affects diverse metabolic pathways mostly related to glucose, pyruvate, sucrose, starch, and chlorophyll a.
KW - Carbohydrate
KW - Metabolic pathway
KW - Microarray
KW - Phosphate
KW - Rice
UR - http://www.scopus.com/inward/record.url?scp=84355162984&partnerID=8YFLogxK
U2 - 10.1016/j.jplph.2011.09.002
DO - 10.1016/j.jplph.2011.09.002
M3 - Article
C2 - 21978493
AN - SCOPUS:84355162984
SN - 0176-1617
VL - 169
SP - 193
EP - 205
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 2
ER -