TY - JOUR
T1 - Transcriptional profiling of the CAM plant Agave salmiana reveals conservation of a genetic program for regeneration
AU - Cervantes-Pérez, Sergio Alan
AU - Espinal-Centeno, Annie
AU - Oropeza-Aburto, Araceli
AU - Caballero-Pérez, Juan
AU - Falcon, Francisco
AU - Aragón-Raygoza, Alejandro
AU - Sánchez-Segura, Lino
AU - Herrera-Estrella, Luis
AU - Cruz-Hernández, Andrés
AU - Cruz-Ramírez, Alfredo
N1 - Funding Information:
This work was supported by: SICES-Guanajuato Jóvenes Investigadores Grant Number: 14-IJ-DPP-Q182-33 , Universidad de La Salle Bajío Investigadores en Consolidación financement and Consejo Nacional de Ciencia y Tecnología, Fronteras de la Ciencia Grants CONACYT FOINS-301 .
Funding Information:
This work was supported by: SICES-Guanajuato J?venes Investigadores Grant Number: 14-IJ-DPP-Q182-33, Universidad de La Salle Baj?o Investigadores en Consolidaci?n financement and Consejo Nacional de Ciencia y Tecnolog?a, Fronteras de la Ciencia Grants CONACYT FOINS-301.
Publisher Copyright:
© 2018
PY - 2018/10/1
Y1 - 2018/10/1
N2 - In plants, the best characterized plant regeneration process is de novo organogenesis. This type of regeneration is characterized by the formation of a multicellular structure called callus. Calli are induced via phytohormone treatment of plant sections. The callus formation in plants like Agave species with Crassulacean Acid Metabolism (CAM) is poorly studied. In this study, we induced callus formation from Agave salmiana leaves and describe cell arrangement in this tissue. Moreover, we determined and analyzed the transcriptional program of calli, as well as those of differentiated root and leaf tissues, by using RNA-seq. We were able to reconstruct 170,844 transcripts of which 40,644 have a full Open Reading Frame (ORF). The global profile obtained by Next Generation Sequencing (NGS) reveals that several callus-enriched protein coding transcripts are orthologs of previously reported factors highly expressed in Arabidopsis calli. At least 62 genes were differentially expressed in Agave calli, 50 of which were up-regulated. Several of these are actively involved in the perception of, and response to, auxin and cytokinin. Not only are these the first results for the A. salmiana callus, but they provide novel data from roots and leaves of this Agave species, one of the largest non-tree plants in nature.
AB - In plants, the best characterized plant regeneration process is de novo organogenesis. This type of regeneration is characterized by the formation of a multicellular structure called callus. Calli are induced via phytohormone treatment of plant sections. The callus formation in plants like Agave species with Crassulacean Acid Metabolism (CAM) is poorly studied. In this study, we induced callus formation from Agave salmiana leaves and describe cell arrangement in this tissue. Moreover, we determined and analyzed the transcriptional program of calli, as well as those of differentiated root and leaf tissues, by using RNA-seq. We were able to reconstruct 170,844 transcripts of which 40,644 have a full Open Reading Frame (ORF). The global profile obtained by Next Generation Sequencing (NGS) reveals that several callus-enriched protein coding transcripts are orthologs of previously reported factors highly expressed in Arabidopsis calli. At least 62 genes were differentially expressed in Agave calli, 50 of which were up-regulated. Several of these are actively involved in the perception of, and response to, auxin and cytokinin. Not only are these the first results for the A. salmiana callus, but they provide novel data from roots and leaves of this Agave species, one of the largest non-tree plants in nature.
KW - Agave salmiana
KW - Callus
KW - Conservation-CAM
KW - RNAseq-cell reprogramming
KW - Regeneration
UR - http://www.scopus.com/inward/record.url?scp=85046742274&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2018.04.018
DO - 10.1016/j.ydbio.2018.04.018
M3 - Article
C2 - 29705332
AN - SCOPUS:85046742274
VL - 442
SP - 28
EP - 39
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -