TY - JOUR
T1 - Transcriptional controls for early bolting and flowering in angelica sinensis
AU - Li, Mengfei
AU - Li, Jie
AU - Wei, Jianhe
AU - Paré, Paul W.
N1 - Funding Information:
Funding: This work was financially supported by National Natural Science Foundation of China (32160083), China Agriculture Research System of MOF and MARA (CARS-21), Gansu Agricultural University (GSCS-2018-1 and Gaufx-02J04), and Key talent projects of Gansu Province (2020RCXM103).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/9
Y1 - 2021/9
N2 - The root of the perennial herb Angelica sinensis is a widely used source for traditional Chinese medicines. While the plant thrives in cool-moist regions of western China, early bolting and flowering (EBF) for young plants significantly reduces root quality and yield. Approaches to inhibit EBF by changes in physiology during the vernalization process have been investigated; however, the mechanism for activating EBF is still limited. Here, transcript profiles for bolted and unbolted plants (BP and UBP, respectively) were compared by transcriptomic analysis, expression levels of candidate genes were validated by qRT-PCR, and the accumulations of gibberellins (GA1, GA4, GA8, GA9 and GA20 ) were also monitored by HPLC-MS/MS. A total of over 72,000 unigenes were detected with ca. 2600 differentially expressed genes (DEGs) observed in the BP compared with UBP. While various signaling pathways participate in flower induction, it is genes associated with floral development and the sucrose pathway that are observed to be coordinated in EBF plants, coherently up-and down-regulating flowering genes that activate and inhibit flowering, respectively. The signature transcripts pattern for the developmental pathways that drive flowering provides insight into the molecular signals that activate plant EBF.
AB - The root of the perennial herb Angelica sinensis is a widely used source for traditional Chinese medicines. While the plant thrives in cool-moist regions of western China, early bolting and flowering (EBF) for young plants significantly reduces root quality and yield. Approaches to inhibit EBF by changes in physiology during the vernalization process have been investigated; however, the mechanism for activating EBF is still limited. Here, transcript profiles for bolted and unbolted plants (BP and UBP, respectively) were compared by transcriptomic analysis, expression levels of candidate genes were validated by qRT-PCR, and the accumulations of gibberellins (GA1, GA4, GA8, GA9 and GA20 ) were also monitored by HPLC-MS/MS. A total of over 72,000 unigenes were detected with ca. 2600 differentially expressed genes (DEGs) observed in the BP compared with UBP. While various signaling pathways participate in flower induction, it is genes associated with floral development and the sucrose pathway that are observed to be coordinated in EBF plants, coherently up-and down-regulating flowering genes that activate and inhibit flowering, respectively. The signature transcripts pattern for the developmental pathways that drive flowering provides insight into the molecular signals that activate plant EBF.
KW - Angelica sinensis
KW - Early bolting and flowering
KW - Gibberellin metabolism
KW - Sucrose metabolism
KW - Transcriptomic analysis
UR - http://www.scopus.com/inward/record.url?scp=85114934491&partnerID=8YFLogxK
U2 - 10.3390/plants10091931
DO - 10.3390/plants10091931
M3 - Article
AN - SCOPUS:85114934491
SN - 2223-7747
VL - 10
JO - Plants
JF - Plants
IS - 9
M1 - 1931
ER -