TY - JOUR
T1 - Tissue-specific and wound-inducible pattern of expression of the mannopine synthase promoter is determined by the interaction between positive and negative cis-regulatory elements
AU - Guevara-García, Arturo
AU - Mosqueda-Cano, Gilberto
AU - Argüello-Astorga, Gerardo
AU - Simpson, June
AU - Herrera-Estrella, Luis
PY - 1993
Y1 - 1993
N2 - In transgenic tobacco plants the regulatory region of the Agrobacterium genes involved in mannopine synthesis (pmas) directs expression in vascular tissues and is induced upon wounding. To identify cis-acting sequences required for the cell-type-specific and wound-inducible expression of this regulatory region, the expression pattern of chimeric genes in which 5′ upstream deletions of the pmas1′ promoter direct the expression of the β-glucuronidase coding sequence, was analyzed in transgenic tobacco plants. It was found that the pmas1′ promoter is specifically expressed in phloem cells and that this tissue-specific pattern of expression is the result of the interaction of at least two negative regulatory elements with a positive element that directs expression in many cell types. It was also found that wound induction is mediated by two different mechanisms, one of them involving a dramatic change in tissue-specific expression.
AB - In transgenic tobacco plants the regulatory region of the Agrobacterium genes involved in mannopine synthesis (pmas) directs expression in vascular tissues and is induced upon wounding. To identify cis-acting sequences required for the cell-type-specific and wound-inducible expression of this regulatory region, the expression pattern of chimeric genes in which 5′ upstream deletions of the pmas1′ promoter direct the expression of the β-glucuronidase coding sequence, was analyzed in transgenic tobacco plants. It was found that the pmas1′ promoter is specifically expressed in phloem cells and that this tissue-specific pattern of expression is the result of the interaction of at least two negative regulatory elements with a positive element that directs expression in many cell types. It was also found that wound induction is mediated by two different mechanisms, one of them involving a dramatic change in tissue-specific expression.
UR - http://www.scopus.com/inward/record.url?scp=0027668871&partnerID=8YFLogxK
U2 - 10.1046/j.1365-313X.1993.04030495.x
DO - 10.1046/j.1365-313X.1993.04030495.x
M3 - Article
C2 - 8220492
AN - SCOPUS:0027668871
SN - 0960-7412
VL - 4
SP - 495
EP - 505
JO - Plant Journal
JF - Plant Journal
IS - 3
ER -