TY - JOUR
T1 - Tissue culture and plant regeneration of blue grama grass, Bouteloua gracilis (H.B.K.) Lag. ex Steud
AU - Aguado-Santacruz, Gerardo Armando
AU - Cabrera-Ponce, José Luis
AU - Olalde-Portugal, Victor
AU - Saánchez-Gonzalez, Ma Rosario
AU - Maárquez-Guzmaán, Judith
AU - Herrera-Estrella, Luis
N1 - Funding Information:
The authors wish to thank the International Foundation for Science for economic support under grant no. C/2741-1. Corrections and comments by Dr June Simpson (CINVESTAV-IPN, Departamento de Ingeneŕõa Genética de Plantas) and Dr Jeffrey Creque in the final manuscript were very valuable. Thanks are also due to Alejandro Mart́õnez, Alfredo Gamboa and Ricardo Wong (Departamento de Biologṍa, Facultad de Ciencias-UNAM) for technical assistance in histological work.
PY - 2001
Y1 - 2001
N2 - As a first step towards applying biotechnology to blue grama, Bouteloua gracilis (H.B.K.) Lag. ex Steud., we have developed a regenerable tissue culture system for this grass. Shoot apices were isolated from 3-d-old seedlings and cultured in 15 different growth regulator formulations combining 2, 4-dichlorophenoxyacetic acid (2, 4-D), Picloram (4-amino-3, 5, 6-trichloropicolinic acid), N6-benzyladenine (BA) or adenine (6-aminopurine). The highest induction of organogenic callus was obtained with formulations containing 1 mg 1-1 (4.52 μM) 2, 4-D plus 0.5 mg 1-1 (2.22 μM) BA, and 2 mg 1-1 (8.88 μM) BA plus 1 mg 1-1 (4.14 μM) Picloram with or without 40 mg 1-1 (296.08 μM) adenine. Lower frequencies of induction were obtained for embryogenic as compared to organogenic callus. The most efficient treatments for induction of embryogenic callus contained 2 mg 1-1 (9.05 μM) 2, 4-D combined with 0.25 (1.11 μM) or 0.50 mg 1-1 (2.22 μM) BA, or 1 mg 1-1 (4.52 μM) 2, 4-D with 0.50 mg 1-1 (2.22 μM) BA. Regeneration was achieved in hormone-free Murashige and Skoog (MS) medium, half-strength MS medium or MS medium plus 1 mg 1-1 (1.44 μM) gibberellic acid. The number of plantlets regenerated per 500 mg callus fresh weight on MS medium ranged from 9 for 2 mg 1-1 (9.05 μM) 2, 4-D to 62.2 for induction medium containing 2 mg 1-1 (8.28 μM) Picloram, 1 mg 1-1 (4.44 μM) BA and 40 mg 1-1 (296.08 μ M) adenine. Regenerated plants grown in soil under greenhouse conditions reached maturity and produced seeds.
AB - As a first step towards applying biotechnology to blue grama, Bouteloua gracilis (H.B.K.) Lag. ex Steud., we have developed a regenerable tissue culture system for this grass. Shoot apices were isolated from 3-d-old seedlings and cultured in 15 different growth regulator formulations combining 2, 4-dichlorophenoxyacetic acid (2, 4-D), Picloram (4-amino-3, 5, 6-trichloropicolinic acid), N6-benzyladenine (BA) or adenine (6-aminopurine). The highest induction of organogenic callus was obtained with formulations containing 1 mg 1-1 (4.52 μM) 2, 4-D plus 0.5 mg 1-1 (2.22 μM) BA, and 2 mg 1-1 (8.88 μM) BA plus 1 mg 1-1 (4.14 μM) Picloram with or without 40 mg 1-1 (296.08 μM) adenine. Lower frequencies of induction were obtained for embryogenic as compared to organogenic callus. The most efficient treatments for induction of embryogenic callus contained 2 mg 1-1 (9.05 μM) 2, 4-D combined with 0.25 (1.11 μM) or 0.50 mg 1-1 (2.22 μM) BA, or 1 mg 1-1 (4.52 μM) 2, 4-D with 0.50 mg 1-1 (2.22 μM) BA. Regeneration was achieved in hormone-free Murashige and Skoog (MS) medium, half-strength MS medium or MS medium plus 1 mg 1-1 (1.44 μM) gibberellic acid. The number of plantlets regenerated per 500 mg callus fresh weight on MS medium ranged from 9 for 2 mg 1-1 (9.05 μM) 2, 4-D to 62.2 for induction medium containing 2 mg 1-1 (8.28 μM) Picloram, 1 mg 1-1 (4.44 μM) BA and 40 mg 1-1 (296.08 μ M) adenine. Regenerated plants grown in soil under greenhouse conditions reached maturity and produced seeds.
KW - Blue grama
KW - Bouteloua gracilis
KW - Organogenesis
KW - Regeneration
KW - Somatic embryogenesis
KW - Tissue culture
UR - http://www.scopus.com/inward/record.url?scp=0034797241&partnerID=8YFLogxK
U2 - 10.1079/IVP2000118
DO - 10.1079/IVP2000118
M3 - Article
AN - SCOPUS:0034797241
SN - 1054-5476
VL - 37
SP - 182
EP - 189
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
IS - 2
ER -