TY - JOUR
T1 - The utility of infrared spectroscopy as a probe of intact tissue
T2 - Determination of carbon monoxide and hemeproteins in blood and heart muscle
AU - Maxwell, John C.
AU - Barlow, Clyde H.
AU - Spallholz, Julian E.
AU - Caughey, Winslow S.
PY - 1974/11/6
Y1 - 1974/11/6
N2 - Infrared methods permit detection of CO within tissue under nearly physiological conditions. The CO stretch bands exhibit frequencies, band widths and intensities characteristic of the particular binding site with areas related to concentrations. For small volumes (< 1 ml) of whole blood the % HbCO as well as certain abnormal Hbs are rapidly determined. In heart muscle, CO bound to cytochrome oxidase, hemoglobin and myoglobin is observed at 1963, 1951 and 1944 cm-1 respectively, frequencies characteristic of the isolated proteins. Infrared methods discriminate among possible CO binding sites (hemeprotein or other) within any intact tissue. Many other infrared active molecules or groups could also be studied in tissue by infrared spectroscopy.
AB - Infrared methods permit detection of CO within tissue under nearly physiological conditions. The CO stretch bands exhibit frequencies, band widths and intensities characteristic of the particular binding site with areas related to concentrations. For small volumes (< 1 ml) of whole blood the % HbCO as well as certain abnormal Hbs are rapidly determined. In heart muscle, CO bound to cytochrome oxidase, hemoglobin and myoglobin is observed at 1963, 1951 and 1944 cm-1 respectively, frequencies characteristic of the isolated proteins. Infrared methods discriminate among possible CO binding sites (hemeprotein or other) within any intact tissue. Many other infrared active molecules or groups could also be studied in tissue by infrared spectroscopy.
UR - http://www.scopus.com/inward/record.url?scp=0016286775&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(74)90557-9
DO - 10.1016/0006-291X(74)90557-9
M3 - Article
C2 - 4374205
AN - SCOPUS:0016286775
SN - 0006-291X
VL - 61
SP - 230
EP - 236
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -