A chimeric gene was constructed encoding the 36-amino acid transit peptide of a chlorophyll a b-binding protein (AB) and 17 amino acids of the mature protein, linked to the amino terminus of neomycin phosphotransferase II (NPT-II). Tobacco plants transformed with this chimeric gene were obtained using the A. tumefaciens-derived transformation system. Analysis of fractions of chloroplasts isolated from these plants showed that NPT-II was present in the stroma, but not in the thylakoid membrane, which is the normal location for the AB.
- chlorophyll a b-binding protein
- chloroplast targeting
- transit peptide