The transit peptide of a chlorophyll a b-binding protein is not sufficient to insert neomycin phosphotransferase II in the thylakoid membrane

G. van den Broeck, A. van Houtven, M. van Montagu, Luis Herrera-Estrella

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A chimeric gene was constructed encoding the 36-amino acid transit peptide of a chlorophyll a b-binding protein (AB) and 17 amino acids of the mature protein, linked to the amino terminus of neomycin phosphotransferase II (NPT-II). Tobacco plants transformed with this chimeric gene were obtained using the A. tumefaciens-derived transformation system. Analysis of fractions of chloroplasts isolated from these plants showed that NPT-II was present in the stroma, but not in the thylakoid membrane, which is the normal location for the AB.

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalPlant Science
Volume58
Issue number2
DOIs
StatePublished - 1988

Keywords

  • chlorophyll a b-binding protein
  • chloroplast targeting
  • transit peptide

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