@article{8247c119e0cf4040b170f456f7204ab8,
title = "The transit peptide of a chlorophyll a b-binding protein is not sufficient to insert neomycin phosphotransferase II in the thylakoid membrane",
abstract = "A chimeric gene was constructed encoding the 36-amino acid transit peptide of a chlorophyll a b-binding protein (AB) and 17 amino acids of the mature protein, linked to the amino terminus of neomycin phosphotransferase II (NPT-II). Tobacco plants transformed with this chimeric gene were obtained using the A. tumefaciens-derived transformation system. Analysis of fractions of chloroplasts isolated from these plants showed that NPT-II was present in the stroma, but not in the thylakoid membrane, which is the normal location for the AB.",
keywords = "chlorophyll a b-binding protein, chloroplast targeting, transit peptide",
author = "{van den Broeck}, G. and {van Houtven}, A. and {van Montagu}, M. and Luis Herrera-Estrella",
note = "Funding Information: We thank A. Cheung and A. Cashmore for plasmids; A. Caplan, P. Schreier and B. Reiss for critical reading of the manuscript; and M. De Cock, K. Spruyt and S. Van Gijsegem for help in its preparation. This work was supported by grants from the {"}ASLK-Kanker-fonds{"}, the Fonds voor Geneeskundig Wetenschappelijk Onderzoek (F.G.W.O. 3.0001.82), and the Services of the Prime Minister (O.O.A. 12.0561.84) to JS and MVM.",
year = "1988",
doi = "10.1016/0168-9452(88)90006-4",
language = "English",
volume = "58",
pages = "171--176",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Publishing",
number = "2",
}