The therapeutic mode of action of 4-aminopyridine in cerebellar ataxia

Karina Alviña, Kamran Khodakhah

Research output: Contribution to journalArticle

120 Scopus citations

Abstract

Episodic ataxia type 2 (EA2) is a hereditary cerebellar ataxia associated with mutations in the P/Q-type voltage-gated calcium (Ca2+) channels. Therapeutic approaches for treatment of EA2 are very limited. Presently, the potassium (K+) channel blocker 4-aminopyridine (4-AP) constitutes the most promising treatment, although its mechanism of action is not understood. Here we show that, in contrast to what is commonly believed, therapeutic concentrations of 4-AP do not increase the inhibitory drive of cerebellar Purkinje cells. Instead, 4-AP restores the severely diminished precision of pacemaking in Purkinje cells of EA2 mutant mice by prolonging the action potential and increasing the action potential afterhyperpolarization. Consistent with this mode of action, the therapeutic efficacy of 4-AP was comparable, and not additive, to chlorzoxazone, an activator of Ca2+-dependent K +channels that also restores the precision of Purkinje cell pacemaking. The likely target of 4-AP at the concentrations used are the K v1 family of K+ channels, possibly the Kv1.5 subtype. Because at higher concentrations 4-AP blocks a large array of K + channels and is a proconvulsant, use of selective Kv1 channel blockers is likely to be a safer substitute for treatment of cerebellar ataxia. Copyright

Original languageEnglish
Pages (from-to)7258-7268
Number of pages11
JournalJournal of Neuroscience
Volume30
Issue number21
DOIs
StatePublished - May 26 2010

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