The role of UV-B light on small RNA activity during grapevine berry development

Sunitha Sukumaran, Rodrigo Loyola, Jose Alcalde, Patricio Arce-Johnson, J. Tomas Matus, Christopher Rock

Research output: Contribution to journalArticlepeer-review

Abstract

UV-B regulation of anthocyanin biosynthesis in vegetative and grapevine berry tissues has been extensively described. However, its relation with UV-B-regulated microRNAs (miRNAs) has not been addressed before in this species. We explored by deep sequencing of small RNA libraries the developmental dynamics and UV-B effects on miRNAs and associated phased small interfering RNA (phasi-RNAs)-producing loci abundances in in vitro-grown plantlets, in field-grown berry skins of cv. Cabernet Sauvignon, and low- and high UV-B fluence treatments of greenhouse-grown berries at several time points around veraison. We observed by RNA blotting a differential effect of low- versus high-fluence UV-B on miR828 abundances (an effector of anthocyanins and UV-absorbing polyphenolics) across berry development, and identified other miRNAs that correlated with miR828 dynamics. The functional significance of the observed UV-coordinated miRNA responses to UV was supported by degradome evidences of AGO-program
Original languageEnglish
Pages (from-to)769–787. doi: 10.1007/s11248-020-00196-w
JournalG3: Genes, Genomes, and Genetics ms #2018-200575/ Genetics Society of America
StatePublished - Mar 2019

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