The presence of lysylpyridinoline in the hypertrophic cartilage of newly hatched chicks

Michael W. Orth; Daniel A. Martinez; Mark E. Cook; Arthur C. Vailas

doi:10.1016/0304-4165(93)90104-G

The presence of lysylpyridinoline in the hypertrophic cartilage of newly hatched chicks

Michael W. Orth, Daniel A. Martinez, Mark E. Cook, Arthur C. Vailas

Animal and Food Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The presence of lysylpyridinoline (LP) as a nonreducible cross-link in appreciable quantities has primarily been limited to the mineralized tissues, bone and dentin. However, the results reported here show that LP is not only present in the hypertrophic cartilage of the tibiotarsus isolated from newly hatched broiler chicks, but it is approx. 4-fold as concentrated as hydroxylysylpyridinoline (HP). Bone and articular cartilage surrounding the hypertrophic cartilage do not contain measurable quantities of LP. Purified LP has a fluorescent scan similar to purified HP and literature values, confirming that we indeed were measuring LP. Also, the cartilage lesion produced by immature chondrocytes from birds with tibial dyschondroplasia had LP but the HP:LP ratio was > 1. Thus, the low HP:LP ratio could be a marker for hypertrophic cartilage in avians.

Original language	English
Pages (from-to)	229-232
Number of pages	4
Journal	BBA - General Subjects
Volume	1157
Issue number	2
DOIs	https://doi.org/10.1016/0304-4165(93)90104-G
State	Published - Jun 11 1993

Keywords

(Chicken)
Collagen
Cross-linking
Hypertrophic cartilage
Tibial dyschondroplasia

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10.1016/0304-4165(93)90104-G

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title = "The presence of lysylpyridinoline in the hypertrophic cartilage of newly hatched chicks",

abstract = "The presence of lysylpyridinoline (LP) as a nonreducible cross-link in appreciable quantities has primarily been limited to the mineralized tissues, bone and dentin. However, the results reported here show that LP is not only present in the hypertrophic cartilage of the tibiotarsus isolated from newly hatched broiler chicks, but it is approx. 4-fold as concentrated as hydroxylysylpyridinoline (HP). Bone and articular cartilage surrounding the hypertrophic cartilage do not contain measurable quantities of LP. Purified LP has a fluorescent scan similar to purified HP and literature values, confirming that we indeed were measuring LP. Also, the cartilage lesion produced by immature chondrocytes from birds with tibial dyschondroplasia had LP but the HP:LP ratio was > 1. Thus, the low HP:LP ratio could be a marker for hypertrophic cartilage in avians.",

keywords = "(Chicken), Collagen, Cross-linking, Hypertrophic cartilage, Tibial dyschondroplasia",

author = "Orth, {Michael W.} and Martinez, {Daniel A.} and Cook, {Mark E.} and Vailas, {Arthur C.}",

note = "Funding Information: The authors would like to thank Bikram Singh and Cheryl Railing for their technical assistance. The research was partially supported by NASA (NAG-2568), Federal Hatch Grant #0302, and the College of Agriculture and Life Sciences at the University of Wisconsin.",

year = "1993",

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doi = "10.1016/0304-4165(93)90104-G",

language = "English",

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pages = "229--232",

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AU - Orth, Michael W.

AU - Martinez, Daniel A.

AU - Cook, Mark E.

AU - Vailas, Arthur C.

N1 - Funding Information: The authors would like to thank Bikram Singh and Cheryl Railing for their technical assistance. The research was partially supported by NASA (NAG-2568), Federal Hatch Grant #0302, and the College of Agriculture and Life Sciences at the University of Wisconsin.

PY - 1993/6/11

Y1 - 1993/6/11

N2 - The presence of lysylpyridinoline (LP) as a nonreducible cross-link in appreciable quantities has primarily been limited to the mineralized tissues, bone and dentin. However, the results reported here show that LP is not only present in the hypertrophic cartilage of the tibiotarsus isolated from newly hatched broiler chicks, but it is approx. 4-fold as concentrated as hydroxylysylpyridinoline (HP). Bone and articular cartilage surrounding the hypertrophic cartilage do not contain measurable quantities of LP. Purified LP has a fluorescent scan similar to purified HP and literature values, confirming that we indeed were measuring LP. Also, the cartilage lesion produced by immature chondrocytes from birds with tibial dyschondroplasia had LP but the HP:LP ratio was > 1. Thus, the low HP:LP ratio could be a marker for hypertrophic cartilage in avians.

AB - The presence of lysylpyridinoline (LP) as a nonreducible cross-link in appreciable quantities has primarily been limited to the mineralized tissues, bone and dentin. However, the results reported here show that LP is not only present in the hypertrophic cartilage of the tibiotarsus isolated from newly hatched broiler chicks, but it is approx. 4-fold as concentrated as hydroxylysylpyridinoline (HP). Bone and articular cartilage surrounding the hypertrophic cartilage do not contain measurable quantities of LP. Purified LP has a fluorescent scan similar to purified HP and literature values, confirming that we indeed were measuring LP. Also, the cartilage lesion produced by immature chondrocytes from birds with tibial dyschondroplasia had LP but the HP:LP ratio was > 1. Thus, the low HP:LP ratio could be a marker for hypertrophic cartilage in avians.

KW - (Chicken)

KW - Collagen

KW - Cross-linking

KW - Hypertrophic cartilage

KW - Tibial dyschondroplasia

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The presence of lysylpyridinoline in the hypertrophic cartilage of newly hatched chicks

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Keywords

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