The new vertebrate CYP1C family: Cloning of new subfamily members and phylogenetic analysis

Celine A.J. Godard; Jared V. Goldstone; Maya R. Said; Richard L. Dickerson; Bruce R. Woodin; John J. Stegeman

doi:10.1016/j.bbrc.2005.03.231

The new vertebrate CYP1C family: Cloning of new subfamily members and phylogenetic analysis

Celine A.J. Godard, Jared V. Goldstone, Maya R. Said, Richard L. Dickerson, Bruce R. Woodin, John J. Stegeman

Environmental Toxicology

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Abstract

Two novel CYP1 genes from teleost fish constituting a new subfamily have been cloned. These paralogous sequences are designated CYP1C1 and CYP1C2. Both genes were initially obtained from untreated scup Stenotomus chrysops tissues by RT-PCR and RACE. Scup CYP1C1 and CYP1C2 code for 524 and 525 amino acids, respectively, and share 80-81% identity at the nucleotide and amino acid levels. Orthologues of CYP1C1 and CYP1C2 were identified in genome databases for other fish species, and both CYP1B1 and CYP1C1 were cloned from zebrafish (Danio rerio). Phylogenetic analysis shows that CYP1Cs and CYP1Bs constitute a sister clade to the CYP1As. Analysis of sequence domains likely to have functional significance suggests that the two CYP1Cs in scup may have catalytic functions and/or substrate specificity that differ from each other and from those of mammalian CYP1Bs or CYP1As. RT-PCR results indicate that CYP1C1 and CYP1C2 are variously expressed in several scup organs.

Original language	English
Pages (from-to)	1016-1024
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	331
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2005.03.231
State	Published - Jun 17 2005

Keywords

CYP1A
CYP1B1
CYP1C
Cytochrome P450
PAH
Planar aromatic hydrocarbons

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10.1016/j.bbrc.2005.03.231

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title = "The new vertebrate CYP1C family: Cloning of new subfamily members and phylogenetic analysis",

abstract = "Two novel CYP1 genes from teleost fish constituting a new subfamily have been cloned. These paralogous sequences are designated CYP1C1 and CYP1C2. Both genes were initially obtained from untreated scup Stenotomus chrysops tissues by RT-PCR and RACE. Scup CYP1C1 and CYP1C2 code for 524 and 525 amino acids, respectively, and share 80-81% identity at the nucleotide and amino acid levels. Orthologues of CYP1C1 and CYP1C2 were identified in genome databases for other fish species, and both CYP1B1 and CYP1C1 were cloned from zebrafish (Danio rerio). Phylogenetic analysis shows that CYP1Cs and CYP1Bs constitute a sister clade to the CYP1As. Analysis of sequence domains likely to have functional significance suggests that the two CYP1Cs in scup may have catalytic functions and/or substrate specificity that differ from each other and from those of mammalian CYP1Bs or CYP1As. RT-PCR results indicate that CYP1C1 and CYP1C2 are variously expressed in several scup organs.",

keywords = "CYP1A, CYP1B1, CYP1C, Cytochrome P450, PAH, Planar aromatic hydrocarbons",

author = "Godard, {Celine A.J.} and Goldstone, {Jared V.} and Said, {Maya R.} and Dickerson, {Richard L.} and Woodin, {Bruce R.} and Stegeman, {John J.}",

note = "Funding Information: This work was supported by EPA Grant R 827102-01-0 and NIH Grants 5 P42-ES07381 and ES04696. J.V.G. is supported by a Ruth L. Kirschstein NRSA Fellowship (F32 ES012794). This is WHOI publication number 11328. ",

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T2 - Cloning of new subfamily members and phylogenetic analysis

AU - Godard, Celine A.J.

AU - Goldstone, Jared V.

AU - Said, Maya R.

AU - Dickerson, Richard L.

AU - Woodin, Bruce R.

AU - Stegeman, John J.

N1 - Funding Information: This work was supported by EPA Grant R 827102-01-0 and NIH Grants 5 P42-ES07381 and ES04696. J.V.G. is supported by a Ruth L. Kirschstein NRSA Fellowship (F32 ES012794). This is WHOI publication number 11328.

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N2 - Two novel CYP1 genes from teleost fish constituting a new subfamily have been cloned. These paralogous sequences are designated CYP1C1 and CYP1C2. Both genes were initially obtained from untreated scup Stenotomus chrysops tissues by RT-PCR and RACE. Scup CYP1C1 and CYP1C2 code for 524 and 525 amino acids, respectively, and share 80-81% identity at the nucleotide and amino acid levels. Orthologues of CYP1C1 and CYP1C2 were identified in genome databases for other fish species, and both CYP1B1 and CYP1C1 were cloned from zebrafish (Danio rerio). Phylogenetic analysis shows that CYP1Cs and CYP1Bs constitute a sister clade to the CYP1As. Analysis of sequence domains likely to have functional significance suggests that the two CYP1Cs in scup may have catalytic functions and/or substrate specificity that differ from each other and from those of mammalian CYP1Bs or CYP1As. RT-PCR results indicate that CYP1C1 and CYP1C2 are variously expressed in several scup organs.

AB - Two novel CYP1 genes from teleost fish constituting a new subfamily have been cloned. These paralogous sequences are designated CYP1C1 and CYP1C2. Both genes were initially obtained from untreated scup Stenotomus chrysops tissues by RT-PCR and RACE. Scup CYP1C1 and CYP1C2 code for 524 and 525 amino acids, respectively, and share 80-81% identity at the nucleotide and amino acid levels. Orthologues of CYP1C1 and CYP1C2 were identified in genome databases for other fish species, and both CYP1B1 and CYP1C1 were cloned from zebrafish (Danio rerio). Phylogenetic analysis shows that CYP1Cs and CYP1Bs constitute a sister clade to the CYP1As. Analysis of sequence domains likely to have functional significance suggests that the two CYP1Cs in scup may have catalytic functions and/or substrate specificity that differ from each other and from those of mammalian CYP1Bs or CYP1As. RT-PCR results indicate that CYP1C1 and CYP1C2 are variously expressed in several scup organs.

KW - CYP1A

KW - CYP1B1

KW - CYP1C

KW - Cytochrome P450

KW - PAH

KW - Planar aromatic hydrocarbons

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The new vertebrate CYP1C family: Cloning of new subfamily members and phylogenetic analysis

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