The mannopine synthase promoter contains vectorial cis-regulatory elements that act as enhancers and silencers

A. Guevara-García, J. López-Bucio, L. Herrera-Estrella

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A 479-bp bi-directional promoter controls the expression of two genes (mas1' and mas2') that encode enzymes for the synthesis of the opine mannopine in plant tissues infected with Agrobacterium tumefaciens. This 5' regulatory region (mas promoter) contains all the cis-acting elements involved in mediating the complex regulatory properties of these genes in plants. Using different mas promoter regions fused to a minimal 35S promoter (35SΔ108), we found that the regulatory properties of these divergent promoters result from the presence of orientation-dependent negative and positive regulatory regions. Some of these elements have the unusual property of acting as enhancers in one orientation and as silencers in the other. Using electrophoretic mobility shift analysis (EMSA), we showed that the functional mas promoter regions identified by fluorometric and histochemical assays for reporter gene activity in transgenic plants have the ability specifically to bind nuclear protein factors from Nicotiana tabacum, Phaseolus vulgaris, Solanum tuberosum, and Arabidopsis thaliana.

Original languageEnglish
Pages (from-to)608-617
Number of pages10
JournalMolecular and General Genetics
Volume262
Issue number4-5
DOIs
StatePublished - 1999

Keywords

  • Agrobacterium tumefaciens
  • Cis-acting elements
  • Negative regulation
  • mas promoter

Fingerprint Dive into the research topics of 'The mannopine synthase promoter contains vectorial cis-regulatory elements that act as enhancers and silencers'. Together they form a unique fingerprint.

  • Cite this