The maize ZmMIEL1 E3 ligase and ZmMYB83 transcription factor proteins interact and regulate the hypersensitive defence response

Rozalynne Samira, Shailesh Karre, Saet Byul Kim, Peter Balint-Kurti

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The plant hypersensitive response (HR), a rapid cell death at the point of pathogenesis, is mediated by nucleotide-binding site, leucine-rich repeat (NLR) resistance proteins (R-proteins) that recognize the presence of specific pathogen-derived proteins. Rp1-D21 is an autoactive maize NLR R-protein that triggers HR spontaneously. We previously mapped loci associated with variation in the strength of HR induced by Rp1-D21. Here we identify the E3 ligase ZmMIEL1 as the causal gene at a chromosome 10 modifier locus. Transient ZmMIEL1 expression in Nicotiana benthamiana reduced HR induced by Rp1-D21, while suppression of ZmMIEL1 expression in maize carrying Rp1-D21 increased HR. ZmMIEL1 also suppressed HR induced by another autoactive NLR, the Arabidopsis R-protein RPM1D505V, in N. benthamiana. We demonstrated that ZmMIEL1 is a functional E3 ligase and that the effect of ZmMIEL1 was dependent on the proteasome but also that levels of Rp1-D2
Original languageEnglish
Pages (from-to)694-709
Number of pages16
JournalMolecular Plant Pathology
Volume22
Issue number6
DOIs
StatePublished - Jun 2021

Keywords

  • hypersensitive response
  • maize
  • proteasome
  • ubiquitin ligase

Fingerprint

Dive into the research topics of 'The maize ZmMIEL1 E3 ligase and ZmMYB83 transcription factor proteins interact and regulate the hypersensitive defence response'. Together they form a unique fingerprint.

Cite this