The Integrin-Adhesome is Required to Maintain Muscle Structure, Mitochondrial ATP Production, and Movement Forces in Caenorhabditis Elegans

Timothy Etheridge, Md Rahman, Christopher J Gaffney, Debra Shaw, Freya Shepherd, Jignesh Magudia, Deepak E Solomon, Thomas Milne, Jerzy Blawzdziewicz, Dumitru Constantin-Teodosiu, Paul L Greenhaff, Siva Vanapalli, Nathaniel J Szewczyk

Research output: Contribution to journalArticle

Abstract

The integrin-adhesome network, which contains >150 proteins, is mechano-transducing and located at discreet positions along the cell-cell and cell-extracellular matrix interface. A small subset of the integrin-adhesome is known to maintain normal muscle morphology. However, the importance of the entire adhesome for muscle structure and function is unknown. We used RNA interference to knock down 113 putative Caenorhabditis elegans homologs constituting most of the mammalian adhesome and 48 proteins known to localize to attachment sites in C. elegans muscle. In both cases, we found >90% of components were required for normal muscle mitochondrial structure and/or proteostasis vs. empty vector controls. Approximately half of these, mainly proteins that physically interact with each other, were also required for normal sarcomere and/or adhesome structure. Next we confirmed that the dystrophy observed in adhesome mutants associates with impaired maximal mitochondrial ATP production (P < 0.0
Original languageEnglish
Pages (from-to)1235-1246
JournalFASEB Journal
DOIs
StatePublished - Apr 2015

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    Etheridge, T., Rahman, M., Gaffney, C. J., Shaw, D., Shepherd, F., Magudia, J., Solomon, D. E., Milne, T., Blawzdziewicz, J., Constantin-Teodosiu, D., Greenhaff, P. L., Vanapalli, S., & Szewczyk, N. J. (2015). The Integrin-Adhesome is Required to Maintain Muscle Structure, Mitochondrial ATP Production, and Movement Forces in Caenorhabditis Elegans. FASEB Journal, 1235-1246. https://doi.org/10.1096/fj.14-259119