The genetics of the repair of 5-azacytidine-mediated DNA damage in the fission yeast Schizosaccharomyces pombe

Vijay Hegde, Ramsay J. McFarlane, Elaine M. Taylor, Clive Price

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We have recently demonstrated that Schizosaccharomyces pombe cells treated with the nucleoside analogue 5-azacytidine (5-azaC) require previously characterised G2 checkpoint mechanisms for survival. Here we present a survey of known DNA repair mutations which defines those genes required for survival in the presence of 5-azaC. Using a combination of single-mutant and epistasis analyses we find that the excision, mismatch and recombinational repair pathways are all required in some degree for the repair of 5-azaC-mediated DNA damage. There are distinct differences in the epistatic interactions of several of the repair mutations with respect to 5-azaC-mediated DNA damage relative to UV-mediated DNA damage.

Original languageEnglish
Pages (from-to)483-492
Number of pages10
JournalMolecular and General Genetics
Volume251
Issue number4
DOIs
StatePublished - 1996

Keywords

  • 5-Azacytidine
  • DNA repair
  • Fission yeast

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