The first intron of the Arabidopsis thaliana gene coding for elongation factor 1β contains an enhancer-like element

Manuel Gidekel, Beatriz Jimenez, Luis Herrera-Estrella

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Genomic and cDNA clones coding for elongation factor-1β (eEF-1β) from Arabidopsis thaliana (At) were isolated and characterized. eEF-1β was found to be encoded by a single-copy At gene. Chimeric genes fusing the promoter and the 5' untranslated region of the At eEF-1β gene to the gus reporter gene were constructed and used to study the expression of this gene in transgenic tobacco plants. Interestingly, it was found that the first intron of this gene is required for high levels of expression. Experiments using chimeric promoters showed that an enhancer-like element is present in the first intron of At eEF-1β. Gel-shift assays were used to demonstrate that this intron is specifically bound by putative transcription factors present in nuclear protein extracts.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalGene
Volume170
Issue number2
DOIs
StatePublished - May 8 1996

Keywords

  • Transcriptional regulation
  • Transgenic plant
  • Translation
  • β-glucuronidase

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