The effects of flow type on aptamer capture in differential mobility cytometry cell separations

Dimitrios Pappas, Yan Liu, Se W Bae, Kelong Wang, Jong-In Hong, Zhi Zhu, Weihong Tan

Research output: Contribution to journalArticle

Abstract

In this work, differential mobility cytometry (DMC) was used to monitor cell separation based on aptamer recognition for target cells. In this device, open-tubular capillaries coated with Sgc8 aptamers were used as affinity chromatography columns for separation. After cells were injected into the columns, oscillating flow was generated to allow for long-term cell adhesion studies. This process was monitored by opti- cal microscopy, and differential imaging was used to analyze the cells as they adhered to the affinity surface. We investigated the capture time, capture efficiency, purity of target and control cells, as well as the reusability of the affinity columns. Capture time for both CCRF-CEM cells and Jurkat T cells was 0.4 ± 0.2 s, which demonstrated the high separation affinity between aptamers and target cells. The cap- ture efficiency for CCRF-CEM cells was 95% and purity was 99% in a cell mixture. With the advantage of both high cell capture efficiency and purity, DMC combine
Original languageEnglish
Pages (from-to)95-100
JournalAnalytica Chimica Acta
StatePublished - 2010

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