TY - JOUR
T1 - The effect of chronic methamphetamine exposure on the hippocampal and olfactory bulb neuroproteomes of rats
AU - Zhu, Rui
AU - Yang, Tianjiao
AU - Kobeissy, Firas
AU - Mouhieddine, Tarek H.
AU - Raad, Mohamad
AU - Nokkari, Amaly
AU - Gold, Mark S.
AU - Wang, Kevin K.
AU - Mechref, Yehia
PY - 2016/4/15
Y1 - 2016/4/15
N2 - Nowadays, drug abuse and addiction are serious public health problems in the USA. Methamphetamine (METH) is one of the most abused drugs and is known to cause brain damage after repeated exposure. In this paper, we conducted a neuroproteomic study to evaluate METH-induced brain protein dynamics, following a two-week chronic regimen of an escalating dose of METH exposure. Proteins were extracted from rat brain hippocampal and olfactory bulb tissues and subjected to liquid chromatography-mass spectrometry (LCMS/ MS) analysis. Both shotgun and targeted proteomic analysis were performed. Protein quantification was initially based on comparing the spectral counts between METH exposed animals and their control counterparts. Quantitative differences were further confirmed through multiple reaction monitoring (MRM) LC-MS/MS experiments. According to the quantitative results, the expression of 18 proteins (11 in the hippocampus and 7 in the olfactory bulb) underwent a significant alteration as a result of exposing rats to METH. 13 of these proteins were up-regulated after METH exposure while 5 were down-regulated. The altered proteins belonging to different structural and functional families were involved in processes such as cell death, inflammation, oxidation, and apoptosis.
AB - Nowadays, drug abuse and addiction are serious public health problems in the USA. Methamphetamine (METH) is one of the most abused drugs and is known to cause brain damage after repeated exposure. In this paper, we conducted a neuroproteomic study to evaluate METH-induced brain protein dynamics, following a two-week chronic regimen of an escalating dose of METH exposure. Proteins were extracted from rat brain hippocampal and olfactory bulb tissues and subjected to liquid chromatography-mass spectrometry (LCMS/ MS) analysis. Both shotgun and targeted proteomic analysis were performed. Protein quantification was initially based on comparing the spectral counts between METH exposed animals and their control counterparts. Quantitative differences were further confirmed through multiple reaction monitoring (MRM) LC-MS/MS experiments. According to the quantitative results, the expression of 18 proteins (11 in the hippocampus and 7 in the olfactory bulb) underwent a significant alteration as a result of exposing rats to METH. 13 of these proteins were up-regulated after METH exposure while 5 were down-regulated. The altered proteins belonging to different structural and functional families were involved in processes such as cell death, inflammation, oxidation, and apoptosis.
UR - http://www.scopus.com/inward/record.url?scp=84994381336&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0151034
DO - 10.1371/journal.pone.0151034
M3 - Article
C2 - 27082425
AN - SCOPUS:84994381336
VL - 11
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 4
M1 - 0151034
ER -