The avocado genome informs deep angiosperm phylogeny, highlights introgressive hybridization, and reveals pathogen-influenced gene space adaptation

Martha Rendón-Anaya, Enrique Ibarra-Laclette, Alfonso Méndez-Bravo, Tianying Lan, Chunfang Zheng, Lorenzo Carretero-Paulet, Claudia Anahí Perez-Torres, Alejandra Chacón-López, Gustavo Hernandez-Guzmán, Tien Hao Chang, Kimberly M. Farr, W. Brad Barbazuk, Srikar Chamala, Marek Mutwil, Devendra Shivhare, David Alvarez-Ponce, Neena Mitter, Alice Hayward, Stephen Fletcher, Julio RozasAlejandro Sánchez Gracia, David Kuhn, Alejandro F. Barrientos-Priego, Jarkko Salojärvi, Pablo Librado, David Sankoff, Alfredo Herrera-Estrella, Victor A. Albert, Luis Herrera-Estrella

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

The avocado, Persea americana, is a fruit crop of immense importance to Mexican agriculture with an increasing demand worldwide. Avocado lies in the anciently diverged magnoliid clade of angiosperms, which has a controversial phylogenetic position relative to eudicots and monocots. We sequenced the nuclear genomes of the Mexican avocado race, P. americana var. drymifolia, and the most commercially popular hybrid cultivar, Hass, and anchored the latter to chromosomes using a genetic map. Resequenc-ing of Guatemalan and West Indian varieties revealed that ∼39% of the Hass genome represents Guatemalan source regions introgressed into a Mexican race background. Some introgressed blocks are extremely large, consistent with the recent origin of the cultivar. The avocado lineage experienced 2 lineage-specific polyploidy events during its evolutionary history. Although gene-tree/ species-tree phylogenomic results are inconclusive, syntenic ortholog distances to other species place avocado as sister to the enormous monocot and eudicot lineages combined. Duplicate genes descending from polyploidy augmented the transcription factor diversity of avocado, while tandem duplicates enhanced the secondary metabolism of the species. Phenylpropanoid biosynthesis, known to be elicited by Colletotrichum (anthracnose) pathogen infection in avocado, is one enriched function among tandems. Furthermore, transcriptome data show that tandem duplicates are significantly up- and down-regulated in response to anthracnose infection, whereas polyploid duplicates are not, supporting the general view that collections of tandem duplicates contribute evolutionarily recent “tuning knobs” in the genome adaptive landscapes of given species.

Original languageEnglish
Pages (from-to)17081-17089
Number of pages9
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number34
DOIs
StatePublished - Aug 20 2019

Keywords

  • Angiosperm phylogeny
  • Avocado genome
  • Genome duplications
  • Genome evolution
  • Phytophthora

Fingerprint

Dive into the research topics of 'The avocado genome informs deep angiosperm phylogeny, highlights introgressive hybridization, and reveals pathogen-influenced gene space adaptation'. Together they form a unique fingerprint.

Cite this