TY - JOUR
T1 - The Arabidopsis ascorbate peroxidase 3 is a peroxisomal membrane-bound antioxidant enzyme and is dispensable for Arabidopsis growth and development
AU - Narendra, Savitha
AU - Venkataramani, Sujatha
AU - Shen, Guoxin
AU - Wang, Jing
AU - Pasapula, Vijaya
AU - Lin, Yun
AU - Kornyeyev, Dmytro
AU - Holaday, A. Scott
AU - Zhang, Hong
N1 - Funding Information:
Biological Resources Center at Ohio State University for the APX3 knockout mutant and the polyubiquitin gene clone. This work was supported by grants from the Texas Advanced Technology Program.
PY - 2006/9
Y1 - 2006/9
N2 - Ascorbate peroxidase (APX) exists as several isoforms that are found in various compartments in plant cells. The cytosolic and chloroplast APXs appear to play important roles in antioxidation metabolism in plant cells, yet the function of peroxisomal APX is not well studied. In this study, the localization of a putative peroxisomal membrane-bound ascorbate peroxidase, APX3 from Arabidopsis, was confirmed by studying the green fluorescent protein (GFP)-APX3 fusion protein in transgenic plants. GFP-APX3 was found to co-localize with a reporter protein that was targeted to peroxisomes by the peroxisomal targeting signal 1. The function of APX3 in Arabidopsis was investigated by analysing an APX3 knockout mutant under normal and several stress conditions. It was found that loss of function in APX3 does not affect Arabidopsis growth and development, suggesting that APX3 may not be an important antioxidant enzyme in Arabidopsis, at least under the conditions that were tested, or the function of APX3 could be compensated by other antioxidant enzymes in plant cells.
AB - Ascorbate peroxidase (APX) exists as several isoforms that are found in various compartments in plant cells. The cytosolic and chloroplast APXs appear to play important roles in antioxidation metabolism in plant cells, yet the function of peroxisomal APX is not well studied. In this study, the localization of a putative peroxisomal membrane-bound ascorbate peroxidase, APX3 from Arabidopsis, was confirmed by studying the green fluorescent protein (GFP)-APX3 fusion protein in transgenic plants. GFP-APX3 was found to co-localize with a reporter protein that was targeted to peroxisomes by the peroxisomal targeting signal 1. The function of APX3 in Arabidopsis was investigated by analysing an APX3 knockout mutant under normal and several stress conditions. It was found that loss of function in APX3 does not affect Arabidopsis growth and development, suggesting that APX3 may not be an important antioxidant enzyme in Arabidopsis, at least under the conditions that were tested, or the function of APX3 could be compensated by other antioxidant enzymes in plant cells.
KW - Antioxidation
KW - Hydrogen peroxide scavenging
KW - Peroxisomal membrane targeting
KW - Reactive oxygen species
KW - Subcellular localization
UR - http://www.scopus.com/inward/record.url?scp=33748993414&partnerID=8YFLogxK
U2 - 10.1093/jxb/erl060
DO - 10.1093/jxb/erl060
M3 - Article
C2 - 16873450
AN - SCOPUS:33748993414
SN - 0022-0957
VL - 57
SP - 3033
EP - 3042
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 12
ER -