Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

Guido Van Den Broeck; Michael P. Timko; Albert P. Kausch; Anthony R. Cashmore; Marc Van Montagu; Luis Herrera-Estrella

doi:10.1038/313358a0

Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

Guido Van Den Broeck, Michael P. Timko, Albert P. Kausch, Anthony R. Cashmore, Marc Van Montagu, Luis Herrera-Estrella

Plant & Soil Science

Research output: Contribution to journal › Article › peer-review

192 Scopus citations

Abstract

Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose l,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.

Original language	English
Pages (from-to)	358-363
Number of pages	6
Journal	Nature
Volume	313
Issue number	6001
DOIs	https://doi.org/10.1038/313358a0
State	Published - 1985

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@article{340230decefd48cbbbe887e1ed6aa9c9,

title = "Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase",

abstract = "Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose l,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.",

author = "{Van Den Broeck}, Guido and Timko, {Michael P.} and Kausch, {Albert P.} and Cashmore, {Anthony R.} and {Van Montagu}, Marc and Luis Herrera-Estrella",

year = "1985",

doi = "10.1038/313358a0",

language = "English",

volume = "313",

pages = "358--363",

journal = "Nature",

issn = "0028-0836",

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T1 - Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

AU - Van Den Broeck, Guido

AU - Timko, Michael P.

AU - Kausch, Albert P.

AU - Cashmore, Anthony R.

AU - Van Montagu, Marc

AU - Herrera-Estrella, Luis

PY - 1985

Y1 - 1985

N2 - Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose l,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.

AB - Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose l,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.

UR - http://www.scopus.com/inward/record.url?scp=0021967344&partnerID=8YFLogxK

U2 - 10.1038/313358a0

DO - 10.1038/313358a0

M3 - Article

C2 - 3969146

AN - SCOPUS:0021967344

SN - 0028-0836

VL - 313

SP - 358

EP - 363

JO - Nature

JF - Nature

IS - 6001

ER -

Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

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