T cell transfer model of chronic colitis: Concepts, considerations, and tricks of the trade

Dmitry V. Ostanin, Jianxiong Bao, Iurii Koboziev, Laura Gray, Sherry A. Robinson-Jackson, Melissa Kosloski-Davidson, V. Hugh Price, Matthew B. Grisham

Research output: Contribution to journalReview article

242 Scopus citations

Abstract

The inflammatory bowel diseases (Crohn's disease; ulcerative colitis) are idiopathic chronic inflammatory disorders of the intestine and/or colon. A major advancement in our understanding of the pathogenesis of these diseases has been the development of mouse models of chronic gut inflammation. One model that has been instrumental in delineating the immunological mechanisms responsible for the induction as well as regulation of intestinal inflammation is the T cell transfer model of chronic colitis. This paper presents a detailed protocol describing the methods used to induce chronic colitis in mice. Special attention is given to the immunological concepts that explain disease pathogenesis in this model, considerations and potential pitfalls in using this model, and finally different "tricks" that we have learned over the past 12 years that have allowed us to develop a more simplified version of this model of experimental IBD.

Original languageEnglish
Pages (from-to)G135-G146
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume296
Issue number2
DOIs
StatePublished - Feb 2009

Keywords

  • Adaptive immune system
  • Cytokines
  • IL-10-deficient mice
  • Inflammation
  • Inflammatory bowel disease
  • T cell trafficking

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    Ostanin, D. V., Bao, J., Koboziev, I., Gray, L., Robinson-Jackson, S. A., Kosloski-Davidson, M., Price, V. H., & Grisham, M. B. (2009). T cell transfer model of chronic colitis: Concepts, considerations, and tricks of the trade. American Journal of Physiology - Gastrointestinal and Liver Physiology, 296(2), G135-G146. https://doi.org/10.1152/ajpgi.90462.2008