TY - JOUR
T1 - T cell transfer model of chronic colitis
T2 - Concepts, considerations, and tricks of the trade
AU - Ostanin, Dmitry V.
AU - Bao, Jianxiong
AU - Koboziev, Iurii
AU - Gray, Laura
AU - Robinson-Jackson, Sherry A.
AU - Kosloski-Davidson, Melissa
AU - Price, V. Hugh
AU - Grisham, Matthew B.
PY - 2009/2
Y1 - 2009/2
N2 - The inflammatory bowel diseases (Crohn's disease; ulcerative colitis) are idiopathic chronic inflammatory disorders of the intestine and/or colon. A major advancement in our understanding of the pathogenesis of these diseases has been the development of mouse models of chronic gut inflammation. One model that has been instrumental in delineating the immunological mechanisms responsible for the induction as well as regulation of intestinal inflammation is the T cell transfer model of chronic colitis. This paper presents a detailed protocol describing the methods used to induce chronic colitis in mice. Special attention is given to the immunological concepts that explain disease pathogenesis in this model, considerations and potential pitfalls in using this model, and finally different "tricks" that we have learned over the past 12 years that have allowed us to develop a more simplified version of this model of experimental IBD.
AB - The inflammatory bowel diseases (Crohn's disease; ulcerative colitis) are idiopathic chronic inflammatory disorders of the intestine and/or colon. A major advancement in our understanding of the pathogenesis of these diseases has been the development of mouse models of chronic gut inflammation. One model that has been instrumental in delineating the immunological mechanisms responsible for the induction as well as regulation of intestinal inflammation is the T cell transfer model of chronic colitis. This paper presents a detailed protocol describing the methods used to induce chronic colitis in mice. Special attention is given to the immunological concepts that explain disease pathogenesis in this model, considerations and potential pitfalls in using this model, and finally different "tricks" that we have learned over the past 12 years that have allowed us to develop a more simplified version of this model of experimental IBD.
KW - Adaptive immune system
KW - Cytokines
KW - IL-10-deficient mice
KW - Inflammation
KW - Inflammatory bowel disease
KW - T cell trafficking
UR - http://www.scopus.com/inward/record.url?scp=59449094473&partnerID=8YFLogxK
U2 - 10.1152/ajpgi.90462.2008
DO - 10.1152/ajpgi.90462.2008
M3 - Review article
C2 - 19033538
AN - SCOPUS:59449094473
SN - 0193-1857
VL - 296
SP - G135-G146
JO - American Journal of Physiology - Gastrointestinal and Liver Physiology
JF - American Journal of Physiology - Gastrointestinal and Liver Physiology
IS - 2
ER -