Synthesis of 13C7-labeled iodoacetanilide and application to quantitative analysis of peptides and a protein by isotope differential mass spectrometry

Satomi Niwayama, Masoud Zabet-Moghaddam, Sadamu Kurono, Hanjoung Cho

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

13C7-Labeled iodoacetanilide has been synthesized for specific labeling of sulfhydryl groups of cysteine residues and has been successfully applied to quantitative analysis of peptides and a commercial protein in combination with 13C-unlabeled iodoacetanilide and a MALDI mass spectrometer. Subsequent tandem mass spectrum analysis revealed that 13C7-labeled iodoacetanilide remained intact during the collision-induced dissociation (CID) conditions.

Original languageEnglish
Pages (from-to)5698-5702
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number19
DOIs
StatePublished - Oct 1 2009

Keywords

  • Cysteine modifier
  • Mass spectrometry
  • Proteomics
  • Quantitative analysis of peptides

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