Synthesis of d-labeled and unlabeled ethyl succinic anhydrides and application to quantitative analysis of peptides by isotope differential mass spectrometry

Satomi Niwayama; Masoud Zabet-Moghaddam; Sadamu Kurono; Pullaiah Kattanguru; Aarif L. Shaikh

doi:10.1016/j.bmcl.2016.08.079

Synthesis of d-labeled and unlabeled ethyl succinic anhydrides and application to quantitative analysis of peptides by isotope differential mass spectrometry

Satomi Niwayama, Masoud Zabet-Moghaddam, Sadamu Kurono, Pullaiah Kattanguru, Aarif L. Shaikh

Center for BioTechnology Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

Ethyl succinic anhydride and its d₅-labeled version have been synthesized and applied to quantitative analysis of peptides in combination with MALDI or ESI mass spectrometry. These modifiers react with amino groups in the N-termini and lysine side chains in proteins, and therefore the combination of these modifiers was shown to be a useful tool for quantification of peptides and hence for proteomics research.

Original language	English
Pages (from-to)	5073-5077
Number of pages	5
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	26
Issue number	20
DOIs	https://doi.org/10.1016/j.bmcl.2016.08.079
State	Published - 2016

Keywords

Amino-group modifiers
Proteomics
Quantitative analysis
Soft ionization mass spectrometry
Stable isotope labeling

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10.1016/j.bmcl.2016.08.079

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title = "Synthesis of d-labeled and unlabeled ethyl succinic anhydrides and application to quantitative analysis of peptides by isotope differential mass spectrometry",

abstract = "Ethyl succinic anhydride and its d5-labeled version have been synthesized and applied to quantitative analysis of peptides in combination with MALDI or ESI mass spectrometry. These modifiers react with amino groups in the N-termini and lysine side chains in proteins, and therefore the combination of these modifiers was shown to be a useful tool for quantification of peptides and hence for proteomics research.",

keywords = "Amino-group modifiers, Proteomics, Quantitative analysis, Soft ionization mass spectrometry, Stable isotope labeling",

author = "Satomi Niwayama and Masoud Zabet-Moghaddam and Sadamu Kurono and Pullaiah Kattanguru and Shaikh, {Aarif L.}",

note = "Funding Information: This work is supported by the Texas Tech University-Texas Tech University Health Sciences Center Joint Initiative Grant and the Akiyama Life Science Foundation Grant. We thank the Center for Biotechnology and Genomics, Texas Tech University for allowing us to use the research facilities. We also thank Professor Ted W. Reid, in the Department of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, for providing us with a peptide. Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd",

year = "2016",

doi = "10.1016/j.bmcl.2016.08.079",

language = "English",

volume = "26",

pages = "5073--5077",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

number = "20",

}

Synthesis of d-labeled and unlabeled ethyl succinic anhydrides and application to quantitative analysis of peptides by isotope differential mass spectrometry. / Niwayama, Satomi; Zabet-Moghaddam, Masoud; Kurono, Sadamu et al.
In: Bioorganic and Medicinal Chemistry Letters, Vol. 26, No. 20, 2016, p. 5073-5077.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Synthesis of d-labeled and unlabeled ethyl succinic anhydrides and application to quantitative analysis of peptides by isotope differential mass spectrometry

AU - Niwayama, Satomi

AU - Zabet-Moghaddam, Masoud

AU - Kurono, Sadamu

AU - Kattanguru, Pullaiah

AU - Shaikh, Aarif L.

N1 - Funding Information: This work is supported by the Texas Tech University-Texas Tech University Health Sciences Center Joint Initiative Grant and the Akiyama Life Science Foundation Grant. We thank the Center for Biotechnology and Genomics, Texas Tech University for allowing us to use the research facilities. We also thank Professor Ted W. Reid, in the Department of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, for providing us with a peptide. Publisher Copyright: © 2016 Elsevier Ltd

PY - 2016

Y1 - 2016

N2 - Ethyl succinic anhydride and its d5-labeled version have been synthesized and applied to quantitative analysis of peptides in combination with MALDI or ESI mass spectrometry. These modifiers react with amino groups in the N-termini and lysine side chains in proteins, and therefore the combination of these modifiers was shown to be a useful tool for quantification of peptides and hence for proteomics research.

AB - Ethyl succinic anhydride and its d5-labeled version have been synthesized and applied to quantitative analysis of peptides in combination with MALDI or ESI mass spectrometry. These modifiers react with amino groups in the N-termini and lysine side chains in proteins, and therefore the combination of these modifiers was shown to be a useful tool for quantification of peptides and hence for proteomics research.

KW - Amino-group modifiers

KW - Proteomics

KW - Quantitative analysis

KW - Soft ionization mass spectrometry

KW - Stable isotope labeling

UR - http://www.scopus.com/inward/record.url?scp=84988927295&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2016.08.079

DO - 10.1016/j.bmcl.2016.08.079

M3 - Article

C2 - 27624079

AN - SCOPUS:84988927295

SN - 0960-894X

VL - 26

SP - 5073

EP - 5077

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 20

ER -

Synthesis of d-labeled and unlabeled ethyl succinic anhydrides and application to quantitative analysis of peptides by isotope differential mass spectrometry

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Keywords

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