TY - JOUR
T1 - Synthesis of d-labeled and unlabeled ethyl succinic anhydrides and application to quantitative analysis of peptides by isotope differential mass spectrometry
AU - Niwayama, Satomi
AU - Zabet-Moghaddam, Masoud
AU - Kurono, Sadamu
AU - Kattanguru, Pullaiah
AU - Shaikh, Aarif L.
N1 - Publisher Copyright:
© 2016 Elsevier Ltd
PY - 2016
Y1 - 2016
N2 - Ethyl succinic anhydride and its d5-labeled version have been synthesized and applied to quantitative analysis of peptides in combination with MALDI or ESI mass spectrometry. These modifiers react with amino groups in the N-termini and lysine side chains in proteins, and therefore the combination of these modifiers was shown to be a useful tool for quantification of peptides and hence for proteomics research.
AB - Ethyl succinic anhydride and its d5-labeled version have been synthesized and applied to quantitative analysis of peptides in combination with MALDI or ESI mass spectrometry. These modifiers react with amino groups in the N-termini and lysine side chains in proteins, and therefore the combination of these modifiers was shown to be a useful tool for quantification of peptides and hence for proteomics research.
KW - Amino-group modifiers
KW - Proteomics
KW - Quantitative analysis
KW - Soft ionization mass spectrometry
KW - Stable isotope labeling
UR - http://www.scopus.com/inward/record.url?scp=84988927295&partnerID=8YFLogxK
U2 - 10.1016/j.bmcl.2016.08.079
DO - 10.1016/j.bmcl.2016.08.079
M3 - Article
C2 - 27624079
AN - SCOPUS:84988927295
SN - 0960-894X
VL - 26
SP - 5073
EP - 5077
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 20
ER -