Synthesis of d-labeled and unlabeled ethyl succinic anhydrides and application to quantitative analysis of peptides by isotope differential mass spectrometry

Satomi Niwayama, Masoud Zabet-Moghaddam, Sadamu Kurono, Pullaiah Kattanguru, Aarif L. Shaikh

Research output: Contribution to journalArticle

Abstract

Ethyl succinic anhydride and its d5-labeled version have been synthesized and applied to quantitative analysis of peptides in combination with MALDI or ESI mass spectrometry. These modifiers react with amino groups in the N-termini and lysine side chains in proteins, and therefore the combination of these modifiers was shown to be a useful tool for quantification of peptides and hence for proteomics research.

Original languageEnglish
Pages (from-to)5073-5077
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume26
Issue number20
DOIs
StatePublished - 2016

Keywords

  • Amino-group modifiers
  • Proteomics
  • Quantitative analysis
  • Soft ionization mass spectrometry
  • Stable isotope labeling

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