TY - JOUR
T1 - Synergistic effects of bacillus amyloliquefaciens (GB03) and water retaining agent on drought tolerance of perennial ryegrass
AU - Su, An Yu
AU - Niu, Shu Qi
AU - Liu, Yuan Zheng
AU - He, Ao Lei
AU - Zhao, Qi
AU - Paré, Paul W.
AU - Li, Meng Fei
AU - Han, Qing Qing
AU - Khan, Sardar Ali
AU - Zhang, Jin Lin
N1 - Funding Information:
Acknowledgments: This research was supported by National Natural Science Foundation of China (grant No. 31222053 and 31172256), Hui-Chun Chin and Tsung-Dao Lee Chinese Undergraduate Research Endowment (JZH0089) and Science and Technology Support Program of Gansu Province, China (1604NKCA077).
Publisher Copyright:
© 2017 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2017/12/11
Y1 - 2017/12/11
N2 - Water retaining agent (WRA) is widely used for soil erosion control and agricultural water saving. Here, we evaluated the effects of the combination of beneficial soil bacterium Bacillus amyloliquefaciens strain GB03 and WRA (the compound is super absorbent hydrogels) on drought tolerance of perennial ryegrass (Lolium perenne L.). Seedlings were subjected to natural drought for maximum 20 days by stopping watering and then rewatered for seven days. Plant survival rate, biomass, photosynthesis, water status and leaf cell membrane integrity were measured. The results showed that under severe drought stress (20-day natural drought), compared to control, GB03, WRA and GB03+WRA all significantly improved shoot fresh weight, dry weight, relative water content (RWC) and chlorophyll content and decreased leaf relative electric conductivity (REC) and leaf malondialdehyde (MDA) content; GB03+WRA significantly enhanced chlorophyll content compared to control and other two treatments. Seven days after rewatering, GB03, WRA and GB03+WRA all significantly enhanced plant survival rate, biomass, RWC and maintained chlorophyll content compared to control; GB03+WRA significantly enhanced plant survival rate, biomass and chlorophyll content compared to control and other two treatments. The results established that GB03 together with water retaining agent promotes ryegrass growth under drought conditions by improving survival rate and maintaining chlorophyll content.
AB - Water retaining agent (WRA) is widely used for soil erosion control and agricultural water saving. Here, we evaluated the effects of the combination of beneficial soil bacterium Bacillus amyloliquefaciens strain GB03 and WRA (the compound is super absorbent hydrogels) on drought tolerance of perennial ryegrass (Lolium perenne L.). Seedlings were subjected to natural drought for maximum 20 days by stopping watering and then rewatered for seven days. Plant survival rate, biomass, photosynthesis, water status and leaf cell membrane integrity were measured. The results showed that under severe drought stress (20-day natural drought), compared to control, GB03, WRA and GB03+WRA all significantly improved shoot fresh weight, dry weight, relative water content (RWC) and chlorophyll content and decreased leaf relative electric conductivity (REC) and leaf malondialdehyde (MDA) content; GB03+WRA significantly enhanced chlorophyll content compared to control and other two treatments. Seven days after rewatering, GB03, WRA and GB03+WRA all significantly enhanced plant survival rate, biomass, RWC and maintained chlorophyll content compared to control; GB03+WRA significantly enhanced plant survival rate, biomass and chlorophyll content compared to control and other two treatments. The results established that GB03 together with water retaining agent promotes ryegrass growth under drought conditions by improving survival rate and maintaining chlorophyll content.
KW - Bacillus amyloliquefaciens
KW - Drought tolerance
KW - Perennial ryegrass
KW - Synergistic effects
KW - Water retaining agent
UR - http://www.scopus.com/inward/record.url?scp=85037855591&partnerID=8YFLogxK
U2 - 10.3390/ijms18122651
DO - 10.3390/ijms18122651
M3 - Article
C2 - 29232909
AN - SCOPUS:85037855591
SN - 1661-6596
VL - 18
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 12
M1 - 2651
ER -