Formation of biofilm on implant surface is one of the majorcauses for port-operative infection. Incorporation ofantimicrobial silver nanoparticles (AgNPs) on implant surfacehas been studied as an effective remedy in recent decades. Thispaper presents a novel AgNPs-titanium composite foilmanufactured through covalent attachment of AgNPs forbiofilm control. This composite foil was fabricated by a seriesof chemical reactions including thiol-derivatization andformation of Ag-S covalent bonds. The film structure andproperties were characterized through scanning electronmicroscopy (SEM), Energy dispersive X-ray Spectroscopy(EDS) and surface profiling. The antimicrobial performancewas examined against gram positive bacterial strainStaphylococcus aureus. Results indicate that the preparedcomposite foil effectively reduced bacterial attachment in shortterm exposure and possess high stability against the release ofnanoparticles. This novel antimicrobial composite material hasa great potential to be used in the future biomedicalinstrumentation.