Sugar beet germination: Phenotypic selection and molecular profiling to identify genes involved in abiotic stress response

J. Mitchell McGrath, Abla Elawady, Dina El-Khishin, Rachel P. Naegele, Kevin M. Carr, Benildo De Los Reyes

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

6 Scopus citations

Abstract

Emergence and stand establishment are critical concerns of sugar beet growers worldwide, and abiotic stresses potentially limit the types of varieties that can be grown productively. This project seeks to develop information that will be useful in selecting and breeding sugar beet for enhanced emergence in saline conditions. We developed a simple bioassay using seeds submerged in salt water to screen 147 germplasm lines for their ability to germinate in 150 mM NaCl as compared with 0.3% H2O2, and used the ratio as a predictor of field emergence potential (FEP). Simultaneously, we surveyed gene expression during salt germination in order to identify potential molecular breeding targets. Expressed Sequence Tags (ESTs) were obtained from 3,119 transcripts of 4-day old seedlings germinated in saline and H2O 2 solutions. Results suggest that 1-carbon metabolism is an important process in stress germinating seedlings. Results also indicated that germplasm differs in germination under salt stress, and in at least one germplasm, differential gene expression is observed. Results from the salt germination assay appear to be consistent with tolerance at the whole plant level, at least for some tested lines. This information will allow subsequent breeding and physiological studies on sugar beet salt tolerance, for instance in identifying highly salt tolerant sugarbeet germplasm that could be used to help remediate saline soils.

Original languageEnglish
Title of host publicationProceedings of the Fourth International Symposium on Seed, Transplant and Stand Establishment of Horticultural Crops
PublisherInternational Society for Horticultural Science
Pages35-49
Number of pages15
ISBN (Print)9789066050594
DOIs
StatePublished - Feb 2008

Publication series

NameActa Horticulturae
Volume782
ISSN (Print)0567-7572

Keywords

  • Bioinformatics
  • Expressed sequence tags
  • Field emergence potential
  • Genomics
  • Salt stress
  • Transcript enrichment

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