Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: Identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli

Leonardo Curatti, Eduardo Folco, Paula Desplats, Gustavo Abratti, Verónica Limones, Luis Herrera-Estrella, Graciela Salerno

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Abstract

The first identification and characterization of a prokaryotic gene (spsA) encoding sucrose-phosphate synthase (SPS) is reported for Synechocystis sp. strain PCC 6803, a unicellular non-nitrogen-fixing cyanobacterium. Comparisons of the deduced amino acid sequence and some relevant biochemical properties of the enzyme with those of plant SPSs revealed important differences in the N-terminal and UDP-glucose binding site regions, substrate specificities, molecular masses, subunit compositions, and regulatory properties.

Original languageEnglish
Pages (from-to)6776-6779
Number of pages4
JournalJournal of Bacteriology
Volume180
Issue number24
DOIs
StatePublished - Dec 1998

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