TY - JOUR
T1 - Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803
T2 - Identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli
AU - Curatti, Leonardo
AU - Folco, Eduardo
AU - Desplats, Paula
AU - Abratti, Gustavo
AU - Limones, Verónica
AU - Herrera-Estrella, Luis
AU - Salerno, Graciela
PY - 1998/12
Y1 - 1998/12
N2 - The first identification and characterization of a prokaryotic gene (spsA) encoding sucrose-phosphate synthase (SPS) is reported for Synechocystis sp. strain PCC 6803, a unicellular non-nitrogen-fixing cyanobacterium. Comparisons of the deduced amino acid sequence and some relevant biochemical properties of the enzyme with those of plant SPSs revealed important differences in the N-terminal and UDP-glucose binding site regions, substrate specificities, molecular masses, subunit compositions, and regulatory properties.
AB - The first identification and characterization of a prokaryotic gene (spsA) encoding sucrose-phosphate synthase (SPS) is reported for Synechocystis sp. strain PCC 6803, a unicellular non-nitrogen-fixing cyanobacterium. Comparisons of the deduced amino acid sequence and some relevant biochemical properties of the enzyme with those of plant SPSs revealed important differences in the N-terminal and UDP-glucose binding site regions, substrate specificities, molecular masses, subunit compositions, and regulatory properties.
UR - http://www.scopus.com/inward/record.url?scp=0032440388&partnerID=8YFLogxK
U2 - 10.1128/jb.180.24.6776-6779.1998
DO - 10.1128/jb.180.24.6776-6779.1998
M3 - Article
C2 - 9852031
AN - SCOPUS:0032440388
SN - 0021-9193
VL - 180
SP - 6776
EP - 6779
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 24
ER -