Structure and Mechanism of a Metal-Sensing Regulatory RNA

Charles E. Dann, Catherine A. Wakeman, Cecelia L. Sieling, Stephanie C. Baker, Irnov Irnov, Wade C. Winkler

Research output: Contribution to journalArticle

241 Scopus citations

Abstract

Organisms maintain the correct balance of intracellular metals primarily through metal-sensing proteins that control transport and storage of the target ion(s). Here, we reveal the basis of metal sensing and genetic control by a metalloregulatory RNA. Our data demonstrate that a previously uncharacterized orphan riboswitch, renamed the "M-box," is a divalent metal-sensing RNA involved in Mg2+ homeostasis. A combination of genetic, biochemical, and biophysical techniques demonstrate that Mg2+ induces a compacted tertiary architecture for M-box RNAs that regulates the accessibility of nucleotides involved in genetic control. Molecular details are provided by crystallographic structure determination of a Mg2+-bound M-box RNA. Given the distribution of this RNA element, it may constitute a common mode for bacterial metal ion regulation, and its discovery suggests the possibility of additional RNA-based metal sensors in modern and primordial organisms.

Original languageEnglish
Pages (from-to)878-892
Number of pages15
JournalCell
Volume130
Issue number5
DOIs
StatePublished - Sep 7 2007

Keywords

  • CELLBIO
  • RNA

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    Dann, C. E., Wakeman, C. A., Sieling, C. L., Baker, S. C., Irnov, I., & Winkler, W. C. (2007). Structure and Mechanism of a Metal-Sensing Regulatory RNA. Cell, 130(5), 878-892. https://doi.org/10.1016/j.cell.2007.06.051