TY - JOUR
T1 - Structure and Mechanism of a Metal-Sensing Regulatory RNA
AU - Dann, Charles E.
AU - Wakeman, Catherine A.
AU - Sieling, Cecelia L.
AU - Baker, Stephanie C.
AU - Irnov, Irnov
AU - Winkler, Wade C.
PY - 2007/9/7
Y1 - 2007/9/7
N2 - Organisms maintain the correct balance of intracellular metals primarily through metal-sensing proteins that control transport and storage of the target ion(s). Here, we reveal the basis of metal sensing and genetic control by a metalloregulatory RNA. Our data demonstrate that a previously uncharacterized orphan riboswitch, renamed the "M-box," is a divalent metal-sensing RNA involved in Mg2+ homeostasis. A combination of genetic, biochemical, and biophysical techniques demonstrate that Mg2+ induces a compacted tertiary architecture for M-box RNAs that regulates the accessibility of nucleotides involved in genetic control. Molecular details are provided by crystallographic structure determination of a Mg2+-bound M-box RNA. Given the distribution of this RNA element, it may constitute a common mode for bacterial metal ion regulation, and its discovery suggests the possibility of additional RNA-based metal sensors in modern and primordial organisms.
AB - Organisms maintain the correct balance of intracellular metals primarily through metal-sensing proteins that control transport and storage of the target ion(s). Here, we reveal the basis of metal sensing and genetic control by a metalloregulatory RNA. Our data demonstrate that a previously uncharacterized orphan riboswitch, renamed the "M-box," is a divalent metal-sensing RNA involved in Mg2+ homeostasis. A combination of genetic, biochemical, and biophysical techniques demonstrate that Mg2+ induces a compacted tertiary architecture for M-box RNAs that regulates the accessibility of nucleotides involved in genetic control. Molecular details are provided by crystallographic structure determination of a Mg2+-bound M-box RNA. Given the distribution of this RNA element, it may constitute a common mode for bacterial metal ion regulation, and its discovery suggests the possibility of additional RNA-based metal sensors in modern and primordial organisms.
KW - CELLBIO
KW - RNA
UR - http://www.scopus.com/inward/record.url?scp=34548253368&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2007.06.051
DO - 10.1016/j.cell.2007.06.051
M3 - Article
C2 - 17803910
AN - SCOPUS:34548253368
VL - 130
SP - 878
EP - 892
JO - Cell
JF - Cell
SN - 0092-8674
IS - 5
ER -