Abstract
The sterol and fatty acid compositions of Epifagus virginiana, which is a nonphotosynthetic flowering plant, have been examined by combined gas liquid chromatography-mass spectrometry (GLC-MS) and nuclear magnetic resonance (NMR). The organism exhibited a typical higher plant Δ5-sterol profile with the C-24 alkyl group oriented "α" for the major sterol Trace amounts of 24-methylenecycloartanol and cycloeucalenol established connection with the first cyclic intermediate (cycloartenol) in photosynthetic plant sterol biosynthesis. In a separate in vitro experiment, excised root and stem tissue were incubated with two labeled triterpenoid precursors, [2-3H] lanosterol and [2-3H]cycloartenol. Both the radioactive compounds were converted to labeled [3H]sitosterol indicating the presence of an active biosynthetic pathway in Epifagus. Characteristic photosynthetic higher plant fatty acids were also present, and the polyenic acid was identified as α-linolenic acid.
Original language | English |
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Pages (from-to) | 274-276 |
Number of pages | 3 |
Journal | Lipids |
Volume | 14 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1979 |