Sterol specificity of the Saccharomyces cerevisiae ERG6 gene product expressed in Escherichia coli

Mylavarapu Venkatramesh; De An Guo; James G. Harman; W. David Nes

doi:10.1007/BF02522922

Sterol specificity of the Saccharomyces cerevisiae ERG6 gene product expressed in Escherichia coli

Mylavarapu Venkatramesh, De An Guo, James G. Harman, W. David Nes

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The ERG6 gene from Saccharomyces cerevisiae has been functionally expressed in Escherichia coli, for the first time, yielding a protein that catalyzes the bisubstrate transfer reaction whereby the reactive methyl group from (S)-adenosyl-L-methionine is transferred stereoselectively to C-24 of the sterol side chain. The structural requirements of sterol in binding and catalysis were similar to the native protein from S. cerevisiae. Inhibition of biomethylation was observed with fecosterol and ergosterol which suggests that ergosterol may function in wild type yeast as a feedback regulator of sterol biosynthesis.

Original language	English
Pages (from-to)	373-377
Number of pages	5
Journal	Lipids
Volume	31
Issue number	4
DOIs	https://doi.org/10.1007/BF02522922
State	Published - Apr 1996

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abstract = "The ERG6 gene from Saccharomyces cerevisiae has been functionally expressed in Escherichia coli, for the first time, yielding a protein that catalyzes the bisubstrate transfer reaction whereby the reactive methyl group from (S)-adenosyl-L-methionine is transferred stereoselectively to C-24 of the sterol side chain. The structural requirements of sterol in binding and catalysis were similar to the native protein from S. cerevisiae. Inhibition of biomethylation was observed with fecosterol and ergosterol which suggests that ergosterol may function in wild type yeast as a feedback regulator of sterol biosynthesis.",

author = "Mylavarapu Venkatramesh and Guo, {De An} and Harman, {James G.} and Nes, {W. David}",

year = "1996",

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AU - Venkatramesh, Mylavarapu

AU - Guo, De An

AU - Harman, James G.

AU - Nes, W. David

PY - 1996/4

Y1 - 1996/4

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AB - The ERG6 gene from Saccharomyces cerevisiae has been functionally expressed in Escherichia coli, for the first time, yielding a protein that catalyzes the bisubstrate transfer reaction whereby the reactive methyl group from (S)-adenosyl-L-methionine is transferred stereoselectively to C-24 of the sterol side chain. The structural requirements of sterol in binding and catalysis were similar to the native protein from S. cerevisiae. Inhibition of biomethylation was observed with fecosterol and ergosterol which suggests that ergosterol may function in wild type yeast as a feedback regulator of sterol biosynthesis.

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JO - Lipids

JF - Lipids

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ER -

Sterol specificity of the Saccharomyces cerevisiae ERG6 gene product expressed in Escherichia coli

Abstract

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