Abstract
[methyl-2H3]Methionine and zymosterol, [27-13C]lanosterol, [24-2H]lanosterol and lanosterol were separately incubated with the sterol auxotroph Saccharomyces cerevisiae strain GL7. Spectral evidence (1H and 13C-NMR) obtained on three different isotopically labeled ergosterol samples indicated that C-28 was derived from AdoMet, H-24 migrated to C-25 and the C-25 hydrogen on (27-)-methyl 13C-labeled ergosterol was introduced from the Re-face to produce the 25R-stereochemistry.
Original language | English |
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Pages (from-to) | 1339-1342 |
Number of pages | 4 |
Journal | Tetrahedron Letters |
Volume | 37 |
Issue number | 9 |
DOIs | |
State | Published - Feb 26 1996 |