Abstract
Using a homogenate prepared from Escherichia coli cells that express the sterol methyl transferase (SMT) gene of Arabidopsis thaliana, migration of the hydrogen atom at C-24 to C-25 from the Re-face of the double bond was demonstrated in the biosynthesis of [27-13C] 24(28)-methylenezymosterol (fecosterol) from [27-13C]zymosterol and the chirality of the C-25 stereocenter (25R) was found to be retained after the stereospecific conversion of [27-13C]24(28)-methylenezymosterol to [27-13C](24(28)Z) -ethylidenecholest-8-en-3β-ol.
Original language | English |
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Pages (from-to) | 6115-6118 |
Number of pages | 4 |
Journal | Tetrahedron Letters |
Volume | 38 |
Issue number | 35 |
DOIs | |
State | Published - Sep 1 1997 |