STCH4/REIL2 Confers Cold Stress Tolerance in Arabidopsis by Promoting rRNA Processing and CBF Protein Translation

Hasi Yu, Xiangfeng Kong, Huan Huang, Wenwu Wu, Junghoon Park, Dae Jin Yun, Byeong ha Lee, Huazhong Shi, Jian Kang Zhu

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Plants respond to cold stress by inducing the expression of transcription factors that regulate downstream genes to confer tolerance to freezing. We screened an Arabidopsis transfer DNA (T-DNA) insertion library and identified a cold-hypersensitive mutant, which we named stch4 (sensitive to chilling 4). STCH4/REIL2 encodes a ribosomal biogenesis factor that is upregulated upon cold stress. Overexpression of STCH4 confers chilling and freezing tolerance in Arabidopsis. The stch4 mutation reduces CBF protein levels and thus delayed the induction of C-repeat-binding factor (CBF) regulon genes. Ribosomal RNA processing is reduced in stch4 mutants, especially under cold stress. STCH4 associates with multiple ribosomal proteins, and these interactions are modulated by cold stress. These results suggest that the ribosome is a regulatory node for cold stress responses and that STCH4 promotes an altered ribosomal composition and functions in low temperatures to facilitate the translation of proteins important for plant growth and survival under cold stress.

Original languageEnglish
Pages (from-to)229-242.e5
JournalCell Reports
Volume30
Issue number1
DOIs
StatePublished - Jan 7 2020

Keywords

  • CBF
  • STCH4
  • cold stress
  • protein translation
  • rRNA processing
  • ribosome

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