Abstract
The breakthrough discovery of RNA interference (RNAi) by Fire and Mello in 1998 has ushered in a new wave of RNA-based technological advances in the life sciences. Small RNAs, namely small interfering RNA (siRNA) and microRNA (miRNA), not only play key roles in down regulating gene expression, controlling growth and development, stress response, and various diseases, but also serve as essential tools for the study of gene functions. In this chapter, we provide an overview of the technological aspects of siRNAs and miRNAs and common methods for studying their functions.
Original language | English |
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Title of host publication | Current Perspectives in microRNAs (miRNA) |
Publisher | Springer Netherlands |
Pages | 17-33 |
Number of pages | 17 |
ISBN (Electronic) | 9781402085338 |
ISBN (Print) | 9781402085321 |
DOIs | |
State | Published - 2008 |
Keywords
- antagomiR
- miRNA
- miRNA profiling
- miRNA sponge
- siRNA
- target mimicry