Small RNA technologies: SiRNA, miRNA, antagomiR, target mimicry, miRNA sponge and miRNA profiling

Guiliang Tang; Yu Xiang; Zhensheng Kang; Venugopal Mendu; Xiaohu Tang; Xiaoyun Jia; Qi Jun Chen; Xiaoqing Tang

doi:10.1007/978-1-4020-8533-8_2

Small RNA technologies: SiRNA, miRNA, antagomiR, target mimicry, miRNA sponge and miRNA profiling

Guiliang Tang, Yu Xiang, Zhensheng Kang, Venugopal Mendu, Xiaohu Tang, Xiaoyun Jia, Qi Jun Chen, Xiaoqing Tang

Fiber and Biopolymer Research Inst

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

The breakthrough discovery of RNA interference (RNAi) by Fire and Mello in 1998 has ushered in a new wave of RNA-based technological advances in the life sciences. Small RNAs, namely small interfering RNA (siRNA) and microRNA (miRNA), not only play key roles in down regulating gene expression, controlling growth and development, stress response, and various diseases, but also serve as essential tools for the study of gene functions. In this chapter, we provide an overview of the technological aspects of siRNAs and miRNAs and common methods for studying their functions.

Original language	English
Title of host publication	Current Perspectives in microRNAs (miRNA)
Publisher	Springer Netherlands
Pages	17-33
Number of pages	17
ISBN (Electronic)	9781402085338
ISBN (Print)	9781402085321
DOIs	https://doi.org/10.1007/978-1-4020-8533-8_2
State	Published - 2008

Keywords

antagomiR
miRNA
miRNA profiling
miRNA sponge
siRNA
target mimicry

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10.1007/978-1-4020-8533-8_2

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abstract = "The breakthrough discovery of RNA interference (RNAi) by Fire and Mello in 1998 has ushered in a new wave of RNA-based technological advances in the life sciences. Small RNAs, namely small interfering RNA (siRNA) and microRNA (miRNA), not only play key roles in down regulating gene expression, controlling growth and development, stress response, and various diseases, but also serve as essential tools for the study of gene functions. In this chapter, we provide an overview of the technological aspects of siRNAs and miRNAs and common methods for studying their functions.",

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author = "Guiliang Tang and Yu Xiang and Zhensheng Kang and Venugopal Mendu and Xiaohu Tang and Xiaoyun Jia and Chen, {Qi Jun} and Xiaoqing Tang",

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T2 - SiRNA, miRNA, antagomiR, target mimicry, miRNA sponge and miRNA profiling

AU - Tang, Guiliang

AU - Xiang, Yu

AU - Kang, Zhensheng

AU - Mendu, Venugopal

AU - Tang, Xiaohu

AU - Jia, Xiaoyun

AU - Chen, Qi Jun

AU - Tang, Xiaoqing

PY - 2008

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AB - The breakthrough discovery of RNA interference (RNAi) by Fire and Mello in 1998 has ushered in a new wave of RNA-based technological advances in the life sciences. Small RNAs, namely small interfering RNA (siRNA) and microRNA (miRNA), not only play key roles in down regulating gene expression, controlling growth and development, stress response, and various diseases, but also serve as essential tools for the study of gene functions. In this chapter, we provide an overview of the technological aspects of siRNAs and miRNAs and common methods for studying their functions.

KW - antagomiR

KW - miRNA

KW - miRNA profiling

KW - miRNA sponge

KW - siRNA

KW - target mimicry

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M3 - Chapter

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SP - 17

EP - 33

BT - Current Perspectives in microRNAs (miRNA)

PB - Springer Netherlands

ER -

Small RNA technologies: SiRNA, miRNA, antagomiR, target mimicry, miRNA sponge and miRNA profiling

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Keywords

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