Small RNA technologies: SiRNA, miRNA, antagomiR, target mimicry, miRNA sponge and miRNA profiling

Guiliang Tang, Yu Xiang, Zhensheng Kang, Venugopal Mendu, Xiaohu Tang, Xiaoyun Jia, Qi Jun Chen, Xiaoqing Tang

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

The breakthrough discovery of RNA interference (RNAi) by Fire and Mello in 1998 has ushered in a new wave of RNA-based technological advances in the life sciences. Small RNAs, namely small interfering RNA (siRNA) and microRNA (miRNA), not only play key roles in down regulating gene expression, controlling growth and development, stress response, and various diseases, but also serve as essential tools for the study of gene functions. In this chapter, we provide an overview of the technological aspects of siRNAs and miRNAs and common methods for studying their functions.

Original languageEnglish
Title of host publicationCurrent Perspectives in microRNAs (miRNA)
PublisherSpringer Netherlands
Pages17-33
Number of pages17
ISBN (Electronic)9781402085338
ISBN (Print)9781402085321
DOIs
StatePublished - 2008

Keywords

  • antagomiR
  • miRNA
  • miRNA profiling
  • miRNA sponge
  • siRNA
  • target mimicry

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