Bioinspired artificial channels can combine high stability with the high permeability and selectivity found in biological channels. They have potential applications in next-generation energy-efficient and stable aqueous separation applications. This study is focused on a new architecture of artificial water channels-peptide-appended pillararenes. This study combined stopped-flow measurement with a newly developed fluorescence correlation spectroscopy technique to, for the first time, successfully determined the single channel water permeability of artificial channels. The peptide-appended pillararene channel permeability was determined to be (2.81±0.11)×10-17 cm3/s per channel or (9.68±0.38)×105 H2O molecules/s per channel. This permeability value is only 2 magnitudes lower than that of natural water channel-aquaporins, when the low cross-sectional area (0.65 nm2) is compared to larger cross sectional area biological water channels (∼10 nm2). This is an orders of magnitude improvement over the first-generation of the artificial water channels reported before and is the fastest one reported so far. This channel was found to have a pore size of approximately 450 Da and showed ion selectivity in the order of NH4 + < Cs+ < Rb+ < K+ < Na+ < Li+ < Cl- as determined by patch clamp studies. The ability to further chemically modify the versatile chemical architecture of the pillararene channels shows promise for further improving water permeability and selectivity.