Single-cell growth kinetics of algae, Chlorela vulgaris

Alim Dewan, Jihye Kim, Swastika S. Bithi, Marci Kerls, Siva A. Vanapalli, M. Nazmul Karim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Traditionally, the growth kinetics of algae is investigated using cell culture equipment thatcontains large number of cells. The kinetic parameters are measured fromrepresentative samples of the cell culture and the measured parametersrepresent the statistical average of all the cells in the samples. In thisstudy, we have investigated the growth kinetics of single-cell of Chlorellavulgaris using droplet based PDMS microfluidic chip. The kinetic parameterswere determined by counting the total number of cells under investigationduring the entire period of the experiment. We found that there is significantheterogeneity in the growth and size of single cells and kinetic parameters aredifferent even if the initial cell numbers are the same. We found that thereis significant heterogeneity in the growth and size of single cells and kineticparameters are different even if the initial cell numbers are the same. Figure1exmplifies the heterogeneity of single-cell growth kinetics. We hypothesizethat the heterogeneity occurs due to the difference in initial cell conditionssuch as the age of the cell, and the size.

Original languageEnglish
Title of host publication1st Annual World Congress on Sustainable Engineering 2011 - Topical Conference at the 2011 AIChE Annual Meeting
PublisherAIChE
Pages27-28
Number of pages2
ISBN (Print)9781618395818
StatePublished - 2011
Event1st Annual World Congress on Sustainable Engineering 2011 - Topical Conference at the 2011 AIChE Annual Meeting - Minneapolis, United States
Duration: Oct 16 2011Oct 21 2011

Publication series

Name1st Annual World Congress on Sustainable Engineering 2011 - Topical Conference at the 2011 AIChE Annual Meeting

Conference

Conference1st Annual World Congress on Sustainable Engineering 2011 - Topical Conference at the 2011 AIChE Annual Meeting
CountryUnited States
CityMinneapolis
Period10/16/1110/21/11

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  • Cite this

    Dewan, A., Kim, J., Bithi, S. S., Kerls, M., Vanapalli, S. A., & Karim, M. N. (2011). Single-cell growth kinetics of algae, Chlorela vulgaris. In 1st Annual World Congress on Sustainable Engineering 2011 - Topical Conference at the 2011 AIChE Annual Meeting (pp. 27-28). (1st Annual World Congress on Sustainable Engineering 2011 - Topical Conference at the 2011 AIChE Annual Meeting). AIChE.