Simultaneous sampling of volatile and non-volatile analytes in beer for fast fingerprinting by extractive electrospray ionization mass spectrometry

Liang Zhu, Zhong Hu, Gerardo Gamez Goytia, Wai Siang Law, HuanWen Chen, ShuiPing Yang, Konstantin Chingin, Roman M. Balabin, Rui Wang, TingTing Zhang, Renato Zenobi

Research output: Contribution to journalArticle


By gently bubbling nitrogen gas through beer, an effervescent beverage, both volatile and non-volatile compounds can be simultaneously sampled in the form of aerosol. This allows for fast (within seconds) fingerprinting by extractive electrospray ionization mass spectrometry (EESI-MS) in both negative and positive ion mode, without the need for any sample pre-treatment such as degassing and dilution. Trace analytes such as volatile esters (e.g., ethyl acetate and isoamyl acetate), free fatty acids (e.g., caproic acid, caprylic acid, and capric acid), semi/non-volatile organic/inorganic acids (e.g., lactic acid), and various amino acids, commonly present in beer at the low parts per million or at sub-ppm levels, were detected and identified based on tandem MS data. Furthermore, the appearance of solvent cluster ions in the mass spectra gives insight into the sampling and ionization mechanisms: aerosol droplets containing semi/non-volatile substances are thought to be generated via bubb
Original languageEnglish
Pages (from-to)405-413
JournalAnalytical and Bioanalytical Chemistry
StatePublished - Sep 2010


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